BMRB Entry 6520
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR6520
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Title: 1H, 15N Chemical shift assignments for recombinant elicitor protein PcF from the oomycete pathogen P. cactorum
Deposition date: 2005-02-21 Original release date: 2009-05-27
Authors: Nicastro, Giuseppe; Orsomando, Giuseppe; Desario, F.; Ferrari, Elena; Manconi, L.; Spisni, Alberto; Ruggeri, Silverio
Citation: Nicastro, G.; Orsomando, Giuseppe; Desario, F.; Ferrari, E.; Manconi, L.; Spisni, Alberto; Ruggeri, Silverio. "The solution structure of the recombinant elicitor protein PcF from the oomycete pathogen P. cactorum displays a helix-loop-helix fold and reveals calcium binding ability" . ., .-..
Assembly members:
elicitor protein Pcf from the oomycete pathogen P. cactorum, polymer, 52 residues, 6214 Da.
Natural source: Common Name: Pichia Pastoris Taxonomy ID: 4922 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Phytophthora cactorum
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
elicitor protein Pcf from the oomycete pathogen P. cactorum: EDPLYCQAIGCPTLYSEANL
AVSKECRDQGKLGDDFHRCC
EEQCGSTTPASA
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 49 |
| 1H chemical shifts | 321 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Pcf monomer | 1 |
Entities:
Entity 1, Pcf monomer 52 residues - 6214 Da.
| 1 | GLU | ASP | PRO | LEU | TYR | CYS | GLN | ALA | ILE | GLY | ||||
| 2 | CYS | PRO | THR | LEU | TYR | SER | GLU | ALA | ASN | LEU | ||||
| 3 | ALA | VAL | SER | LYS | GLU | CYS | ARG | ASP | GLN | GLY | ||||
| 4 | LYS | LEU | GLY | ASP | ASP | PHE | HIS | ARG | CYS | CYS | ||||
| 5 | GLU | GLU | GLN | CYS | GLY | SER | THR | THR | PRO | ALA | ||||
| 6 | SER | ALA |
Samples:
sample_1: Pcf monomer, [U-15N], 0.65 mM
conditions_1: pH: 5.0; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D-TOCSY-HSQC | not available | not available | not available |
| 3D-NOESY-HSQC | not available | not available | not available |
| 2D-NOESY | not available | not available | not available |
| 2D-TOSCY | not available | not available | not available |
| 2D-HSQC-15N | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Varian INOVA 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts