BMRB Entry 6562
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6562
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: A novel cGUUAg tetraloop structure with a conserved yYNMGg-type backbone conformation from cloverleaf 1 of bovine enterovirus 1 RNA PubMed: 15814817
Deposition date: 2005-03-18 Original release date: 2005-04-19
Authors: Ihle, Yvonne; Ohlenschlager, Oliver; Duchardt, Elke; Ramachandran, Ramadurai; Gorlach, Matthias
Citation: Ihle, Yvonne; Ohlenschlager, Oliver; Hafner, Sabine; Duchardt, Elke; Zacharias, Martin; Seitz, Simone; Zell, Roland; Ramachandran, Ramadurai; Gorlach, Matthias. "A novel cGUUAg tetraloop structure with a conserved yYNMGg-type backbone conformation from cloverleaf 1 of bovine enterovirus 1 RNA" Nucleic Acids Res. 33, 2003-2011 (2005).
Assembly members:
D-loop from cloverleaf 1 of bovine enterovirus 1 RNA, polymer, 18 residues, Formula weight is not available
Natural source: Common Name: bovine enterovirus 1 Taxonomy ID: 12064 Superkingdom: Viruses Kingdom: not available Genus/species: Enterovirus bovine enterovirus 1
Experimental source: Production method: enzymatic semisynthesis
Entity Sequences (FASTA):
D-loop from cloverleaf 1 of bovine enterovirus 1 RNA: GGCGUUCGUUAGAACGUC
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 13C chemical shifts | 197 |
| 15N chemical shifts | 93 |
| 31P chemical shifts | 16 |
| coupling constants | 17 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | BEVSLDap | 1 |
Entities:
Entity 1, BEVSLDap 18 residues - Formula weight is not available
| 1 | G | G | C | G | U | U | C | G | U | U | ||||
| 2 | A | G | A | A | C | G | U | C |
Samples:
sample_1: D-loop from cloverleaf 1 of bovine enterovirus 1 RNA, [U-15N], 1.0 mM; H2O 90%; D2O 10%; KH2PO4/K2HPO4 10 mM; KCl 40 mM; EDTA 0.2 mM
sample_2: D-loop from cloverleaf 1 of bovine enterovirus 1 RNA, [U-13C; U-15N], 0.9 mM; H2O 90%; D2O 10%; KH2PO4/K2HPO4 10 mM; KCl 40 mM; EDTA 0.2 mM
sample_3: D-loop from cloverleaf 1 of bovine enterovirus 1 RNA, [U-13C; U-15N], 0.9 mM; D2O 99.99%; KH2PO4/K2HPO4 10 mM; KCl 40 mM; EDTA 0.2 mM
sample_cond_1: ionic strength: 50 mM; pH: 6.2; pressure: 1 atm; temperature: 298 K
sample_cond_2: ionic strength: 50 mM; pH: 6.2; pressure: 1 atm; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| (1H,15N)-HSQC | not available | not available | not available |
| (1H,15N)-CPMG-NOESY | not available | not available | not available |
| (1H,1H)-H5(C5C4N)H | not available | not available | not available |
| (1H,1H)-HCCNH-TOCSY | not available | not available | not available |
| (1H,1H)-HCCH-TOCSY (A) | not available | not available | not available |
| (1H,1H)-HCCH-COSY | not available | not available | not available |
| (1H,15N)-2bond-HSQC | not available | not available | not available |
| (1H,13C)-H(N)CO | not available | not available | not available |
| (1H,13C)-HSQC | not available | not available | not available |
| 3D (1H,1H,13C)-HCCH-COSY | not available | not available | not available |
| 3D (1H,1H,13C)-HCCH-TOCSY | not available | not available | not available |
| (1H,15N)-HCN | not available | not available | not available |
| 3D (1H,1H,13C)-NOESY-HSQC | not available | not available | not available |
| (1H,31P)-HCP-CCH-TOCSY | not available | not available | not available |
| (1H,1H)-NOESY | not available | not available | not available |
| HNN-COSY | not available | not available | not available |
| (1H,13C)-HCCH-E.COSY | not available | not available | not available |
Software:
VNMR v6.1, rev. c - collection, processing
XEASY v1.3.9 - data analysis
CYANA v1.0.6 - structure solution
FOUND vimplemented in CYANA-1.0.6 - structure solution
OPAL v2.6 - refinement
NMR spectrometers:
- Varian INOVA 750 MHz
- Varian INOVA 600 MHz