BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules 		Member of WWPDB

BMRB Entry 6580

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6580
MolProbity Validation Chart

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Title: assignment of mouse socs3 by NMR   PubMed: 16302975

Deposition date: 2005-04-04 Original release date: 2005-12-16

Authors: Babon, Jeffrey; Yao, Shenggen; deSouza, David; Harrison, Christopher; Fabri, Louis; Liepinsch, Edvards; Otting, Gottfried; Baca, Manuel; Norton, Raymond

Citation: Babon, Jeffrey; Yao, Shenggen; deSouza, David; Harrison, Christopher; Fabri, Louis; Liepinsh, Edvards; Scrofani, Sergio; Baca, Manuel; Norton, Raymond. "Secondary structure assignment of mouse SOCS3 by NMR defines the domain boundaries and identifies an unstructured insertion in the SH2 domain."  FEBS J. 272, 6120-6130 (2005).

Assembly members:
mouse socs3, polymer, 164 residues, Formula weight is not available
gp130 phosphopeptide, polymer, 15 residues, Formula weight is not available

Natural source:   Common Name: domestic mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
mouse socs3: LKTFSSKSEYQLVVNAVRKL QESGFYWSAVTGGEANLLLS AEPAGTFLIRDSSDQRHFFT LSVKTQSGTKNLRIQCEGGS FSLQSDPRSTQPVPRFDCVL KLVHHYMPPPGTPSFSLPPT EPSSEVPEQPPAQALPGSTP KRAYYIYSGGEKIPLVLSRP LSSN
gp130 phosphopeptide: STASTVEXSTVVHSG

Data sets:
Data typeCount
13C chemical shifts478
15N chemical shifts146
1H chemical shifts755

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1mouse socs31
2gp130 phosphopeptide2

Entities:

Entity 1, mouse socs3 164 residues - Formula weight is not available

1   LEULYSTHRPHESERSERLYSSERGLUTYR
2   GLNLEUVALVALASNALAVALARGLYSLEU
3   GLNGLUSERGLYPHETYRTRPSERALAVAL
4   THRGLYGLYGLUALAASNLEULEULEUSER
5   ALAGLUPROALAGLYTHRPHELEUILEARG
6   ASPSERSERASPGLNARGHISPHEPHETHR
7   LEUSERVALLYSTHRGLNSERGLYTHRLYS
8   ASNLEUARGILEGLNCYSGLUGLYGLYSER
9   PHESERLEUGLNSERASPPROARGSERTHR
10   GLNPROVALPROARGPHEASPCYSVALLEU
11   LYSLEUVALHISHISTYRMETPROPROPRO
12   GLYTHRPROSERPHESERLEUPROPROTHR
13   GLUPROSERSERGLUVALPROGLUGLNPRO
14   PROALAGLNALALEUPROGLYSERTHRPRO
15   LYSARGALATYRTYRILETYRSERGLYGLY
16   GLULYSILEPROLEUVALLEUSERARGPRO
17   LEUSERSERASN

Entity 2, gp130 phosphopeptide 15 residues - Formula weight is not available

1   SERTHRALASERTHRVALGLUPTRSERTHR
2   VALVALHISSERGLY

Samples:

sample_1: mouse socs3, [U-13C; U-15N], 0.4 – 0.5 mM; gp130 phosphopeptide, [U-13C; U-15N], 0.4 – 0.5 mM; water mM; glutamic acid 50 mM; arginine 50 mM

sample_2: mouse socs3, [U-13C], 0.4 – 0.5 mM; gp130 phosphopeptide, [U-13C], 0.4 – 0.5 mM; water mM; glutamic acid 50 mM; arginine 50 mM

sample_3: mouse socs3, [U-15N], 0.4 – 0.5 mM; gp130 phosphopeptide, [U-15N], 0.4 – 0.5 mM; water mM; glutamic acid 50 mM; arginine 50 mM

sample_4: mouse socs30.4 – 0.5 mM; gp130 phosphopeptide0.4 – 0.5 mM; water mM; glutamic acid 50 mM; arginine 50 mM

conditions_1: pH: 6.7; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N-HSQCnot availablenot availableconditions_1
13C-HSQCnot availablenot availableconditions_1
13C-NOESY-HSQCnot availablenot availableconditions_1
13C-HNCOnot availablenot availableconditions_1
HNCAnot availablenot availableconditions_1
CBCACONHnot availablenot availableconditions_1
HN(CA)COnot availablenot availableconditions_1
HCCH-TOCSYnot availablenot availableconditions_1
HCCH-COSYnot availablenot availableconditions_1
15N-HSQC-NOESYnot availablenot availableconditions_1
2D 1H NOESYnot availablenot availableconditions_1
1H TOCSYnot availablenot availableconditions_1

Software:

No software information available

NMR spectrometers:

  • . . 500 MHz
  • . . 600 MHz
  • . . 800 MHz

Related Database Links:

PDB
DBJ BAC32977 BAE23929 BAE29797 BAE30668 BAE31222
EMBL CAB56083 CAM19105
GB AAB62403 AAC26223 AAH52031 AAK60601 EDL34646
REF NP_031733 NP_446017
SP O35718 O88583

Download simulated HSQC data in one of the following formats:
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SPARKY: Backbone or all simulated shifts