BMRB Entry 6589
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6589
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Chromo Domain of cpSRP43 PubMed: 16183644
Deposition date: 2005-04-12 Original release date: 2007-02-05
Authors: Vaithiyalingam, Sivaraja; Thallapuranam, Suresh Kumar; Philominathan, Leena; Henry, Ralph; Yu, Chin
Citation: Sivaraja, Vaithiyalingam; Suresh Kumar, Thallapuranam; Leena, Philominathan; Chang, Anne; Vidya, Chitturi; Goforth, Robyn; Rajalingam, Dakshinamurthy; Arvind, Kannan; Ye, Jian-Liang; Chou, Jonathan; Henry, Ralph; Yu, Chin. "Three-dimensional solution structures of the chromodomains of cpSRP43" J. Biol. Chem. 280, 41465-41471 (2005).
Assembly members:
Chromo 1, polymer, 47 residues, Formula weight is not available
Natural source: Common Name: thale cress Taxonomy ID: 3702 Superkingdom: Eubacteria Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Chromo 1: GSGEVNKIIGSRTAGEGAME
YLIEWKDGHSPSWVPSSYIA
ADVVSEY
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 107 |
| 15N chemical shifts | 44 |
| 1H chemical shifts | 237 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Chromo domain 1 of cpSRP43 | 1 |
Entities:
Entity 1, Chromo domain 1 of cpSRP43 47 residues - Formula weight is not available
| 1 | GLY | SER | GLY | GLU | VAL | ASN | LYS | ILE | ILE | GLY | ||||
| 2 | SER | ARG | THR | ALA | GLY | GLU | GLY | ALA | MET | GLU | ||||
| 3 | TYR | LEU | ILE | GLU | TRP | LYS | ASP | GLY | HIS | SER | ||||
| 4 | PRO | SER | TRP | VAL | PRO | SER | SER | TYR | ILE | ALA | ||||
| 5 | ALA | ASP | VAL | VAL | SER | GLU | TYR |
Samples:
CD1_-_Chromo_1_domain: CD1 - Chromo domain 1, [U-15N; U-13C], 0.75 ± 0.01 mM; phosphate buffer 100 mM; NaCl 100 mM
CD1_sample: pH: 6.4; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N_HSQC (example) | CD1_-_Chromo_1_domain | not available | CD1_sample |
| 1H 2D NOESY | CD1_-_Chromo_1_domain | not available | CD1_sample |
| 1H 2D TOCSY | CD1_-_Chromo_1_domain | not available | CD1_sample |
| HNCA | CD1_-_Chromo_1_domain | not available | CD1_sample |
| HNCOCA | CD1_-_Chromo_1_domain | not available | CD1_sample |
| CBCACONH | CD1_-_Chromo_1_domain | not available | CD1_sample |
| 3D 15N NOESY-HSQC | CD1_-_Chromo_1_domain | not available | CD1_sample |
Software:
XWIN NMR v3.5, Bruker -
NMR spectrometers:
- Bruker DMX 700 MHz
Related Database Links:
| PDB |
Download simulated HSQC data in one of the following formats:
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