BMRB Entry 6600
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6600
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Title: Solution Structure of Bacillus subtilis s-BCCP apo-form PubMed: 16699181
Deposition date: 2005-04-14 Original release date: 2007-01-26
Authors: Cui, Gaofeng; Xia, Bin
Citation: Cui, Gaofeng; Nan, Beiyan; Hu, Jicheng; Wang, Yiping; Jin, Changwen; Xia, Bin. "Identification and solution structures of a single-domain biotin/lipoyl attachment protein from bacillus subtilis" J. Biol. Chem. 281, 20598-20607 (2006).
Assembly members:
Biotin Carboxyl Carrier Protein, polymer, 72 residues, Formula weight is not available
Natural source: Common Name: Bacillus subtilis Taxonomy ID: 1423 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus subtilis
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Biotin Carboxyl Carrier Protein: TVSIQMAGNLWKVHVKAGDQ
IEKGQEVAILESMKMEIPIV
ADRSGIVKEVKKKEGDFVNE
GDVLLELSNSTQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 325 |
| 15N chemical shifts | 78 |
| 1H chemical shifts | 532 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Biotin Carboxyl Carrier Protein | 1 |
Entities:
Entity 1, Biotin Carboxyl Carrier Protein 72 residues - Formula weight is not available
| 1 | THR | VAL | SER | ILE | GLN | MET | ALA | GLY | ASN | LEU | ||||
| 2 | TRP | LYS | VAL | HIS | VAL | LYS | ALA | GLY | ASP | GLN | ||||
| 3 | ILE | GLU | LYS | GLY | GLN | GLU | VAL | ALA | ILE | LEU | ||||
| 4 | GLU | SER | MET | LYS | MET | GLU | ILE | PRO | ILE | VAL | ||||
| 5 | ALA | ASP | ARG | SER | GLY | ILE | VAL | LYS | GLU | VAL | ||||
| 6 | LYS | LYS | LYS | GLU | GLY | ASP | PHE | VAL | ASN | GLU | ||||
| 7 | GLY | ASP | VAL | LEU | LEU | GLU | LEU | SER | ASN | SER | ||||
| 8 | THR | GLN |
Samples:
sample_1: Biotin Carboxyl Carrier Protein, [U-13C; U-15N], 1.2 mM; phosphate buffer 50 mM; H2O 90%; D2O 10%
sample_cond_1: ionic strength: 150 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | not available | not available | not available |
| 3D 15N-separated NOESY | not available | not available | not available |
Software:
xwinnmr v3.5 - collection
NMRPipe v2.1 - processing
NMRView v5 - data analysis
CYANA v1.0.6 - structure solution
AMBER v7.0 - refinement
NMR spectrometers:
- Bruker AVANCE 600 MHz
Related Database Links:
| BMRB | 6599 |
| PDB | |
| DBJ | BAI85513 BAM52478 BAM58054 GAK81515 |
| EMBL | CAX52627 CEI57017 CEJ77441 |
| GB | ADV92730 AEP91001 AFQ57759 AGE63668 AGG61199 |
| REF | WP_003245519 WP_014476962 WP_014479957 WP_015383786 WP_031600576 |
| SP | C0H419 |
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