BMRB Entry 6699
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6699
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Title: Backbone 1H, 13C, 15N and heavy atoms Chemical Shift Assignments for La3+ monosubstituted calbindin D9k PubMed: 16518694
Deposition date: 2005-06-22 Original release date: 2006-04-27
Authors: Balayssac, Stephane; Jimenez, Beatriz; Piccioli, Mario
Citation: Balayssac, Stephane; Jimenez, Beatriz; Piccioli, Mario. "Assignment Strategy for Fast Relaxing Signals: Complete Aminoacid Identification in Thulium Substituted Calbindin D(9K)" J. Biomol. NMR 34, 63-73 (2006).
Assembly members:
Calcium Protein, polymer, 75 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.
LA, non-polymer, 138.905 Da.
Natural source: Common Name: Cow Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos taurus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Calcium Protein: KSPEELKGIFEKYAAKEGDP
NQLSKEELKLLLQTEFPSLL
KGMSTLDELFEELDKNGDGE
VSFEEFQVLVKKISQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 256 |
| 15N chemical shifts | 77 |
| 1H chemical shifts | 159 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Calbindin D9k | 1 |
| 2 | CALCIUM (II) ION | 2 |
| 3 | LANTHANUM (III) ION | 3 |
Entities:
Entity 1, Calbindin D9k 75 residues - Formula weight is not available
| 1 | LYS | SER | PRO | GLU | GLU | LEU | LYS | GLY | ILE | PHE | ||||
| 2 | GLU | LYS | TYR | ALA | ALA | LYS | GLU | GLY | ASP | PRO | ||||
| 3 | ASN | GLN | LEU | SER | LYS | GLU | GLU | LEU | LYS | LEU | ||||
| 4 | LEU | LEU | GLN | THR | GLU | PHE | PRO | SER | LEU | LEU | ||||
| 5 | LYS | GLY | MET | SER | THR | LEU | ASP | GLU | LEU | PHE | ||||
| 6 | GLU | GLU | LEU | ASP | LYS | ASN | GLY | ASP | GLY | GLU | ||||
| 7 | VAL | SER | PHE | GLU | GLU | PHE | GLN | VAL | LEU | VAL | ||||
| 8 | LYS | LYS | ILE | SER | GLN |
Entity 2, CALCIUM (II) ION - Ca - 40.078 Da.
| 1 | CA |
Entity 3, LANTHANUM (III) ION - La - 138.905 Da.
| 1 | LA |
Samples:
sample_1: Calbindin D9k, [U-95% 13C; U-98% 15N], 1 mM; CALCIUM (II) ION 1 mM; LANTHANUM (III) ION 1 mM
conditions_1: pH: 6.0; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H,15N-HSQC | sample_1 | not available | conditions_1 |
| 1H,13C-HSQC | sample_1 | not available | conditions_1 |
| HNCO | sample_1 | not available | conditions_1 |
| HNCA | sample_1 | not available | conditions_1 |
| CBCACONH | sample_1 | not available | conditions_1 |
| 1D 13C | sample_1 | not available | conditions_1 |
| 13C-13C COSY | sample_1 | not available | conditions_1 |
| CACO | sample_1 | not available | conditions_1 |
| CBCACO | sample_1 | not available | conditions_1 |
| CON | sample_1 | not available | conditions_1 |
| 1D 15N | sample_1 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Bruker Avance 900 MHz
- Bruker Avance 700 MHz
- Bruker Avance 500 MHz
- Bruker Avance 400 MHz
Related Database Links:
| PDB | |
| BMRB | 15594 16340 16758 247 325 326 327 4581 5207 6697 753 939 940 |
| GB | AAA30420 AAA72542 AAI18481 ADO85633 |
| PRF | 0707237A:PDB=3ICB |
| REF | NP_776682 XP_004021986 XP_005701114 XP_005888771 XP_005982038 |
| SP | P02633 |
| TPG | DAA12577 |
Download simulated HSQC data in one of the following formats:
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