BMRB Entry 6720
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6720
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution Conformation of alpha-conotoxin PIA PubMed: 16289101
Deposition date: 2005-06-30 Original release date: 2008-07-16
Authors: Chi, S.-W.; Kim, D.-H.; Olivera, B.; Mclntosh, J.; Han, K.-H.
Citation: Chi, S.-W.; Lee, S.-H.; Kim, D.-H.; Kim, J.-S.; Olivera, B.; Mclntosh, J.; Han, K.-H.. "Solution structure of alpha-conotoxin PIA, a novel antagonist of alpha6 subunit containing nicotinic acetylcholine receptors" Biochem. Biophys. Res. Commun. 338, 1990-1997 (2005).
Assembly members:
Alpha-conotoxin PIA, polymer, 19 residues, Formula weight is not available
Natural source: Common Name: Conus purpurascens Taxonomy ID: 41690 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Conus purpurascens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Alpha-conotoxin PIA: RDPCCSNPVCTVHNPQICX
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 90 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Alpha-conotoxin PIA | 1 |
Entities:
Entity 1, Alpha-conotoxin PIA 19 residues - Formula weight is not available
| 1 | ARG | ASP | PRO | CYS | CYS | SER | ASN | PRO | VAL | CYS | ||||
| 2 | THR | VAL | HIS | ASN | PRO | GLN | ILE | CYS | NH2 |
Samples:
sample_1: Alpha-conotoxin PIA 10 mM; H2O 90%; D2O 10%
sample_cond_1: ionic strength: 0 M; pH: 3.92; pressure: 1 atm; temperature: 283 K
sample_cond_2: ionic strength: 0 M; pH: 3.92; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | sample_1 | not available | not available |
| 2D TOCSY | sample_1 | not available | not available |
| DQF-COSY | sample_1 | not available | not available |
Software:
VNMR v6,1B - collection
FELIX v98.0 - structure solution
CNS v1.1 - refinement
NMR spectrometers:
- Varian INOVA 600 MHz