BMRB Entry 6731

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for BOFC
Published: 2007-01-29

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6731

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for BOFC PubMed: 16049010

Deposition date: 2005-07-18 Original release date: 2007-01-29

Authors: Patterson, Hayley; Brannigan, James; Cutting, Simon; Wilson, Keith; Wilkinson, Anthony; Ab, Eiso; Diercks, Tammo; Folkers, Gert; de Jong, Rob; Truffault, Vincent

Citation: Patterson, Hayley; Brannigan, James; Cutting, Simon; Wilson, Keith; Wilkinson, Anthony; Ab, Eiso; Diercks, Tammo; Folkers, Gert; de Jong, Rob; Truffault, Vincent; Kaptein, Rob. "The structure of BofC, an inter-compartmental signalling factor in sporulation in Bacillus" J. Biol. Chem. 280, 36214-36220 (2005).

Assembly members:
BofC, polymer, 140 residues, Formula weight is not available

Natural source: Common Name: Bacillus subtilis Taxonomy ID: 1423 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus subtilis

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):
BofC: AEVEHYEPLQVHVQLEKVYL DGDVSIEHKHEKVFSMDDFW AAYAGWTLVEQKKGYVLFRK QMDDISPLSKVNGYIGVSDN GVISTFHGRPEPASEPIQSF FQIDLERLESHMQKNLLKGI PFRTKAEFEDVIEHMKTYSG

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
13C chemical shifts	617
15N chemical shifts	140
1H chemical shifts	958

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	BofC	1

Entities:

Entity 1, BofC 140 residues - Formula weight is not available

1	ALA	GLU	VAL	GLU	HIS	TYR	GLU	PRO	LEU	GLN
2	VAL	HIS	VAL	GLN	LEU	GLU	LYS	VAL	TYR	LEU
3	ASP	GLY	ASP	VAL	SER	ILE	GLU	HIS	LYS	HIS
4	GLU	LYS	VAL	PHE	SER	MET	ASP	ASP	PHE	TRP
5	ALA	ALA	TYR	ALA	GLY	TRP	THR	LEU	VAL	GLU
6	GLN	LYS	LYS	GLY	TYR	VAL	LEU	PHE	ARG	LYS
7	GLN	MET	ASP	ASP	ILE	SER	PRO	LEU	SER	LYS
8	VAL	ASN	GLY	TYR	ILE	GLY	VAL	SER	ASP	ASN
9	GLY	VAL	ILE	SER	THR	PHE	HIS	GLY	ARG	PRO
10	GLU	PRO	ALA	SER	GLU	PRO	ILE	GLN	SER	PHE
11	PHE	GLN	ILE	ASP	LEU	GLU	ARG	LEU	GLU	SER
12	HIS	MET	GLN	LYS	ASN	LEU	LEU	LYS	GLY	ILE
13	PRO	PHE	ARG	THR	LYS	ALA	GLU	PHE	GLU	ASP
14	VAL	ILE	GLU	HIS	MET	LYS	THR	TYR	SER	GLY

Samples:

sample_one: BofC, [U-15N; U-13C], 0.5 mM; H2O 90%; D2O 10%; NaPO4 20 mM; EDTA 1 mM

condition_one: pH: 6.0; pressure: 1013 hPa; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
not available	sample_one	not available	condition_one

Software:

No software information available

NMR spectrometers:

unknown unknown 0 MHz

PDB	2BW2
DBJ	BAI86261 BAM53209 BAM58851 GAK80383
EMBL	CAA63621 CAB14735 CAB75329 CCU59263 CEI58004
GB	ADM38725 ADV93564 AEP87620 AEP91767 AFI29291
REF	NP_390653 WP_003222663 WP_003229715 WP_003237539 WP_010331022
SP	O05391

Biological Magnetic Resonance Data Bank