BMRB Entry 6753
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6753
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Title: Backbone 1H,13C, and 15N chemical shift assignments for the PH1 domain of alpha-syntrophin PubMed: 16252003
Deposition date: 2005-07-27 Original release date: 2007-01-29
Authors: Yan, Jing; Wen, Wenyu; Xu, Weiguang; Long, Jia-fu; Adams, Marvin; Froehner, Stanley; Zhang, Mingjie
Citation: Yan, Jing; Wen, Wenyu; Xu, Weiguang; Long, Jia-fu; Adams, Marvin; Froehner, Stanley; Zhang, Mingjie. "Structure of the split PH domain and distinct lipid-binding properties of the PH-PDZ supramodule of alpha-syntrophin." EMBO J. 24, 3985-3995 (2005).
Assembly members:
split PH domain, polymer, 178 residues, Formula weight is not available
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
split PH domain: ASGRRAPRTGLLELRCGAGS
GAGGERWQRVLLSLAEDALT
VSPADGEPGPEPEPAQLNGA
AEPGAAPPQLPEALLLQREV
SPYFKNSAGGTSVGWDSPPA
SPLQRQPSSPGPQPRNLSEA
KHVSLKMAYVSRRCTPTDPE
PRYLEICAADGQDAVFLRAK
DEASARSWAGAIQAQIGT
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 483 |
| 15N chemical shifts | 162 |
| 1H chemical shifts | 1066 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | split PH domain | 1 |
Entities:
Entity 1, split PH domain 178 residues - Formula weight is not available
| 1 | ALA | SER | GLY | ARG | ARG | ALA | PRO | ARG | THR | GLY | ||||
| 2 | LEU | LEU | GLU | LEU | ARG | CYS | GLY | ALA | GLY | SER | ||||
| 3 | GLY | ALA | GLY | GLY | GLU | ARG | TRP | GLN | ARG | VAL | ||||
| 4 | LEU | LEU | SER | LEU | ALA | GLU | ASP | ALA | LEU | THR | ||||
| 5 | VAL | SER | PRO | ALA | ASP | GLY | GLU | PRO | GLY | PRO | ||||
| 6 | GLU | PRO | GLU | PRO | ALA | GLN | LEU | ASN | GLY | ALA | ||||
| 7 | ALA | GLU | PRO | GLY | ALA | ALA | PRO | PRO | GLN | LEU | ||||
| 8 | PRO | GLU | ALA | LEU | LEU | LEU | GLN | ARG | GLU | VAL | ||||
| 9 | SER | PRO | TYR | PHE | LYS | ASN | SER | ALA | GLY | GLY | ||||
| 10 | THR | SER | VAL | GLY | TRP | ASP | SER | PRO | PRO | ALA | ||||
| 11 | SER | PRO | LEU | GLN | ARG | GLN | PRO | SER | SER | PRO | ||||
| 12 | GLY | PRO | GLN | PRO | ARG | ASN | LEU | SER | GLU | ALA | ||||
| 13 | LYS | HIS | VAL | SER | LEU | LYS | MET | ALA | TYR | VAL | ||||
| 14 | SER | ARG | ARG | CYS | THR | PRO | THR | ASP | PRO | GLU | ||||
| 15 | PRO | ARG | TYR | LEU | GLU | ILE | CYS | ALA | ALA | ASP | ||||
| 16 | GLY | GLN | ASP | ALA | VAL | PHE | LEU | ARG | ALA | LYS | ||||
| 17 | ASP | GLU | ALA | SER | ALA | ARG | SER | TRP | ALA | GLY | ||||
| 18 | ALA | ILE | GLN | ALA | GLN | ILE | GLY | THR |
Samples:
sample_1: split PH domain, [U-13C; U-15N], 1 mM
sample_2: split PH domain, [U-15N], 1 mM
conditions_1: pH: 7; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCO | not available | not available | not available |
| HNCA | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
| 3D 15N-separated NOESY | not available | not available | not available |
| 3D 15C-separated NOESY | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Varian Inova 750 MHz
Related Database Links:
| PDB |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts