BMRB Entry 6776
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6776
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Title: Assignment of 1H, 15N and 13C resonances of the bacterial protein YFHJ PubMed: 16698547
Deposition date: 2005-08-15 Original release date: 2006-06-27
Authors: Pastore, Chiara; Kelly, Geoff; Adinolfi, Salvatore; McCormick, John; Pastore, Annalisa
Citation: Pastore, Chiara; Adinolfi, Salvatore; Huynen, Martjin; Rybin, Vladimir; Martin, Stephen; Mayer, Mathias; Bukau, Bernds; Pastore, Annalisa. "YfhJ, a Molecular Adaptor in Iron-Sulfur Cluster Formation or a Frataxin-like Protein?" Structure 14, 857-867 (2006).
Assembly members:
YFHJ, polymer, 66 residues, 7731.46 Da.
Natural source: Common Name: Escherichia Coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
YFHJ: MGLKWTDSREIGEALYDAYP
DLDPKTVRFTDMHQWICDLE
DFDDDPQASNEKILEAILLV
WLDEAE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 300 |
| 15N chemical shifts | 69 |
| 1H chemical shifts | 474 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | YFHJ | 1 |
Entities:
Entity 1, YFHJ 66 residues - 7731.46 Da.
| 1 | MET | GLY | LEU | LYS | TRP | THR | ASP | SER | ARG | GLU | ||||
| 2 | ILE | GLY | GLU | ALA | LEU | TYR | ASP | ALA | TYR | PRO | ||||
| 3 | ASP | LEU | ASP | PRO | LYS | THR | VAL | ARG | PHE | THR | ||||
| 4 | ASP | MET | HIS | GLN | TRP | ILE | CYS | ASP | LEU | GLU | ||||
| 5 | ASP | PHE | ASP | ASP | ASP | PRO | GLN | ALA | SER | ASN | ||||
| 6 | GLU | LYS | ILE | LEU | GLU | ALA | ILE | LEU | LEU | VAL | ||||
| 7 | TRP | LEU | ASP | GLU | ALA | GLU |
Samples:
sample_1: YFHJ, [U-98% 15N], 0.5 mM; phosphate 10 mM; NaCl 50%; H20 90%; D2O 10%
sample_2: YFHJ, [U-99% 13C; U-98 15N], 0.6 mM; phosphate 10 mM; NaCl 50 mM; H2O 90%; D2O 10%
conditions_1: pH: 6.5; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H_13C_NOESY-HSQC | not available | not available | conditions_1 |
| 1H_15N_NOESY-HSQC | not available | not available | conditions_1 |
| 1H15N_TOCSY-HSQC | not available | not available | conditions_1 |
| 1H15N_HSQC | not available | not available | conditions_1 |
| 1H13C_HSQC | not available | not available | conditions_1 |
| HCCH_TOCSY | not available | not available | conditions_1 |
| CBCANH | not available | not available | conditions_1 |
| CBCACONH | not available | not available | conditions_1 |
| HNCA | not available | not available | conditions_1 |
| HNCOCA | not available | not available | conditions_1 |
| HNCO | not available | not available | conditions_1 |
| HNHA | not available | not available | conditions_1 |
Software:
XEASY - Assignment
NMRDraw - Data Processing
NMRPipe - Data Processing
NMR spectrometers:
- Varian INOVA 600 MHz
- Varian UNITY-plus 500 MHz
- Varian INOVA 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAA16414 BAB36813 BAG78334 BAI26769 BAI31798 |
| EMBL | CAP76976 CAQ32897 CAQ88192 CAQ99415 CAR03966 |
| GB | AAC75577 AAG57638 AAN44070 AAN81499 AAP17895 |
| REF | NP_289081 NP_311417 NP_417019 NP_708363 NP_754931 |
| SP | P0C0L9 P0C0M0 P0C0M1 Q8FF46 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts