BMRB Entry 6805
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6805
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Title: Letter to the Editor: NMR assignments of the free and bound-state components of an anti-idiotypic affibody complex PubMed: 16456703
Deposition date: 2005-09-01 Original release date: 2006-03-10
Authors: Dogan, Jakob; Lendel, Christofer; Hard, Torleif
Citation: Dogan, Jakob; Lendel, Christofer; Hard, Torleif. "NMR Assignments of the Free and Bound-state Protein Components of an Anti-idiotypic Affibody Complex" J. Biomol. NMR 36, 13-13 (2006).
Assembly members:
anti-Ztaq, polymer, 58 residues, 6419.3 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
anti-Ztaq: VDNKFNKERVIAIGEIMRLP
NLNSLQVVAFINSLRDDPSQ
SANLLAEAKKLNDAQAPK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 247 |
| 15N chemical shifts | 66 |
| 1H chemical shifts | 434 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | anti-Ztaq | 1 |
Entities:
Entity 1, anti-Ztaq 58 residues - 6419.3 Da.
| 1 | VAL | ASP | ASN | LYS | PHE | ASN | LYS | GLU | ARG | VAL | ||||
| 2 | ILE | ALA | ILE | GLY | GLU | ILE | MET | ARG | LEU | PRO | ||||
| 3 | ASN | LEU | ASN | SER | LEU | GLN | VAL | VAL | ALA | PHE | ||||
| 4 | ILE | ASN | SER | LEU | ARG | ASP | ASP | PRO | SER | GLN | ||||
| 5 | SER | ALA | ASN | LEU | LEU | ALA | GLU | ALA | LYS | LYS | ||||
| 6 | LEU | ASN | ASP | ALA | GLN | ALA | PRO | LYS |
Samples:
sample_1: anti-Ztaq, [U-13C; U-15N], 1.5 mM; Potassium Phosphate 20 mM; Sodium Chloride 50 mM; Sodium Azide 0.01%; D2O 10 % v/v
conditions_1: pH*: 6.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| 1H-13C HSQC | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| CBCANH | not available | not available | not available |
| HNCO | not available | not available | not available |
| HN(CA)CO | not available | not available | not available |
| HCCH-COSY | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
| (H)CC(CO)NH | not available | not available | not available |
| 15N-NOESY HSQC | not available | not available | not available |
| 15N-TOCSY HSQC | not available | not available | not available |
| 13C-NOESY HSQC | not available | not available | not available |
Software:
ANSIG for WINDOWS - assignment, Data analysis
XWIN-NMR - processing
NMRPipe - processing
NMR spectrometers:
- BRUKER AVANCE 600 MHz
- BRUKER AVANCE 500 MHz
- VARIAN INOVA 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts