BMRB Entry 6846
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6846
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Title: NMR structural analysis of Nop10p from Saccharomyces cerevisiae PubMed: 16286935
Deposition date: 2005-09-29 Original release date: 2005-12-28
Authors: Hamma, T.; Reichow, S.; Varani, G.; Ferre-D'Amare, A.
Citation: Hamma, T.; Reichow, S.; Varani, G.; Ferre-D'Amare, A.. "The Cbf5-Nop10 complex is a molecular bracket that organizes box H/ACA RNPs" Nat. Struct. Mol. Biol. 12, 1101-1107 (2005).
Assembly members:
H/ACA ribonucleoprotein complex subunit 3, polymer, 63 residues, Formula weight is not available
Natural source: Common Name: baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota not available
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
H/ACA ribonucleoprotein complex subunit 3: HLMYTLGPDGKRIYTLKKVT
ESGEITKSAHPARFSPDDKY
SRQRVTLKKRFGLVPGQHHH
HHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 234 |
| 15N chemical shifts | 58 |
| 1H chemical shifts | 415 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | H/ACA ribonucleoprotein complex subunit 3 | 1 |
Entities:
Entity 1, H/ACA ribonucleoprotein complex subunit 3 63 residues - Formula weight is not available
| 1 | HIS | LEU | MET | TYR | THR | LEU | GLY | PRO | ASP | GLY | ||||
| 2 | LYS | ARG | ILE | TYR | THR | LEU | LYS | LYS | VAL | THR | ||||
| 3 | GLU | SER | GLY | GLU | ILE | THR | LYS | SER | ALA | HIS | ||||
| 4 | PRO | ALA | ARG | PHE | SER | PRO | ASP | ASP | LYS | TYR | ||||
| 5 | SER | ARG | GLN | ARG | VAL | THR | LEU | LYS | LYS | ARG | ||||
| 6 | PHE | GLY | LEU | VAL | PRO | GLY | GLN | HIS | HIS | HIS | ||||
| 7 | HIS | HIS | HIS |
Samples:
sample_1: H/ACA ribonucleoprotein complex subunit 3 1 mM; Sodium Acetate 50 mM; KCl 100 mM; H2O 90%; D2O 10%
sample_2: H/ACA ribonucleoprotein complex subunit 3 1 mM; Sodium Acetate 50 mM; KCl 100 mM; D2O 100%
sample_3: H/ACA ribonucleoprotein complex subunit 3, [U-15N], 1 mM; Sodium Acetate 50 mM; KCl 100 mM; H2O 90%; D2O 10%
sample_4: H/ACA ribonucleoprotein complex subunit 3, [U-13C; U-15N], 1 mM; Sodium Acetate 50 mM; KCl 100 mM; H2O 90%; D2O 10%
sample_cond_1: ionic strength: 100 mM; pH: 5.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | not available | not available | not available |
| 3D 15N-separated NOESY | not available | not available | not available |
| 3D 13C-separated NOESY | not available | not available | not available |
Software:
CYANA vv. 2.0, Guntert, P. - refinement
NMRPipe vv. 2.3, Delaglio, F. - processing
SPARKY vv. 3.110, Goddard. T.D. - data analysis
TALOS vv. 2003.027.13.05, Cornilescu, G. - data analysis
NMR spectrometers:
- Varian INOVA 800 MHz
Related Database Links:
| PDB | |
| DBJ | GAA23788 |
| EMBL | CAY80079 |
| GB | AAS56874 AHY77771 EDN62310 EDV09121 EEU04743 |
| PIR | S78745 |
| REF | NP_058135 XP_011103755 |
| SP | Q6Q547 |
| TPG | DAA06766 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts