BMRB Entry 6850
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6850
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Title: First FF domain of the PRP40 yeast protein PubMed: 16253993
Deposition date: 2005-10-05 Original release date: 2007-01-29
Authors: Gasch, Alexander; Wiesner, Silke; Martin-Malpartida, Pau; Ramirez-Espain, Ximena; Ruiz, Lidia; Macias, Maria
Citation: Gasch, Alexander; Wiesner, Silke; Martin-Malpartida, Pau; Ramirez-Espain, Ximena; Ruiz, Lidia; Macias, Maria. "The structure of Prp40 FF1 domain and its interaction with the crn-TPR1 motif of CLF1 gives a new insight into the binding mode of FF domains." J. Biol. Chem. 281, 356-364 (2006).
Assembly members:
PRP40 first FF domain, polymer, 59 residues, Formula weight is not available
Natural source: Common Name: Baker's Yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
PRP40 first FF domain: GAMEAEKEFITMLKENQVDS
TWSFSRIISELGTRDPRYWM
VDDDPLWKKEMFEKYLSNR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 209 |
| 15N chemical shifts | 65 |
| 1H chemical shifts | 387 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | First FF domain of Prp40 | 1 |
Entities:
Entity 1, First FF domain of Prp40 59 residues - Formula weight is not available
| 1 | GLY | ALA | MET | GLU | ALA | GLU | LYS | GLU | PHE | ILE | ||||
| 2 | THR | MET | LEU | LYS | GLU | ASN | GLN | VAL | ASP | SER | ||||
| 3 | THR | TRP | SER | PHE | SER | ARG | ILE | ILE | SER | GLU | ||||
| 4 | LEU | GLY | THR | ARG | ASP | PRO | ARG | TYR | TRP | MET | ||||
| 5 | VAL | ASP | ASP | ASP | PRO | LEU | TRP | LYS | LYS | GLU | ||||
| 6 | MET | PHE | GLU | LYS | TYR | LEU | SER | ASN | ARG |
Samples:
15N: PRP40 first FF domain, [U-15N], 1 mM; Na Phosphate 20 mM; NaCl 100 mM; H2O 90%; D2O 10%
13C: PRP40 first FF domain, [U-13C; U-15N], 1 mM; Na Phosphate 20 mM; NaCl 100 mM; H2O 90%; D2O 10%
13CD2O: PRP40 first FF domain, [U-13C; U-15N], 1 mM; Na Phosphate 20 mM; NaCl 100 mM; D2O 100%
conditions_1: pressure: 1 atm; temperature: 285 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N edited NOESY | not available | not available | conditions_1 |
| 13C edited NOESY | not available | not available | conditions_1 |
Software:
xwinnmr v3.6, Bruker Biospin AG - Acquisition
NMRPipe, Delaglio F. - processing
CNS v1.1, Brunger A.T. -
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| PDB | |
| DBJ | GAA24712 |
| EMBL | CAA81847 CAY81071 |
| GB | AAB24902 AHY76231 EDN59896 EDV13075 EDZ70922 |
| REF | NP_012913 |
| SP | P33203 |
| TPG | DAA09144 |
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