BMRB Entry 6857
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6857
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Title: Solution structure of an immunodominant epitope of myelin basic protein (MBP) - Conformational dependence on environment of an intrinsically unstructured protein PubMed: 16420483
Deposition date: 2005-10-09 Original release date: 2006-04-20
Authors: Fares, Christophe; Libich, David; Harauz, George
Citation: Fares, Christophe; Libich, David; Harauz, George. "Solution NMR structure of an immunodominant epitope of myelin basic protein" FEBS J. 273, 601-614 (2006).
Assembly members:
FF2, polymer, 18 residues, 2141.4 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
FF2: QDENPVVHFFKNIVTPRT
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 84 |
| 1H chemical shifts | 391 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | FF2 | 1 |
Entities:
Entity 1, FF2 18 residues - 2141.4 Da.
| 1 | GLN | ASP | GLU | ASN | PRO | VAL | VAL | HIS | PHE | PHE | ||||
| 2 | LYS | ASN | ILE | VAL | THR | PRO | ARG | THR |
Samples:
sample_1: FF2, no, 2 mM; KCl, no, 100 mM
sample_2: FF2 5 mM; TFE, [U-2H], 30%
sample_3: FF2 1 mM; DPC, [U-2H], 100 mM
conditions_1: pH: 6.5; temperature: 298 K
conditions_2: pH: 6.5; temperature: 300 K
conditions_3: pH: 6.5; temperature: 318 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D-TOCSY (using DIPSI-2) | not available | not available | not available |
| 2D-NOESY | not available | not available | not available |
| 1H-13C HSQC (natural abundance) | not available | not available | not available |
Software:
XWINNMR v3.5, Bruker - processing
SPARKY v3.111, UCSF NMR Lab - assignment
ARIA v2.0, Jens Linge, Michael Nilges - Structure calculation/refinement
CNS v2.0, Jens Linge, Michael Nilges - Structure calculation/refinement
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| BMRB | 15131 18520 19186 20062 6100 |
| PDB | |
| DBJ | BAB23830 BAC37705 BAE28256 BAE87162 BAE87443 |
| EMBL | CAA10804 CAA10805 CAA10806 CAA10807 CAA35179 |
| GB | AAA37720 AAA39496 AAA39497 AAA39499 AAA39500 |
| PRF | 0411226A 1003243A 1102236A |
| REF | NP_001001546 NP_001020252 NP_001020261 NP_001020263 NP_001020272 |
| SP | P02686 P02687 P02688 P04370 P06906 |
| TPG | DAA16031 |