BMRB Entry 6867
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6867
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Title: Solution structure of micelle-bound fusion domain of HIV-1 gp41 PubMed: 16331977
Deposition date: 2005-10-20 Original release date: 2005-12-28
Authors: Jaroniec, C.; Kaufman, J.; Stahl, S.; Viard, M.; Blumenthal, R.; Wingfield, P.; Bax, A.
Citation: Jaroniec, C.; Kaufman, J.; Stahl, S.; Viard, M.; Blumenthal, R.; Wingfield, P.; Bax, A.. "Structure and Dynamics of Micelle-Associated Human Immunodeficiency Virus gp41 Fusion Domain" Biochemistry 44, 16167-16180 (2005).
Assembly members:
Envelope polyprotein GP160, polymer, 39 residues, Formula weight is not available
Natural source: Common Name: HIV-1 Taxonomy ID: 11676 Superkingdom: Viruses Kingdom: not available Genus/species: Lentivirus HIV-1
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Envelope polyprotein GP160: PAVGIGALFLGFLGAAGSTM
GAASMTLTVQADYKDDDDK
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 110 |
15N chemical shifts | 38 |
1H chemical shifts | 81 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Envelope polyprotein GP160 | 1 |
Entities:
Entity 1, Envelope polyprotein GP160 39 residues - Formula weight is not available
1 | PRO | ALA | VAL | GLY | ILE | GLY | ALA | LEU | PHE | LEU | ||||
2 | GLY | PHE | LEU | GLY | ALA | ALA | GLY | SER | THR | MET | ||||
3 | GLY | ALA | ALA | SER | MET | THR | LEU | THR | VAL | GLN | ||||
4 | ALA | ASP | TYR | LYS | ASP | ASP | ASP | ASP | LYS |
Samples:
sample_1: Envelope polyprotein GP160, [U-2H; U-13C; U-15N], 0.7 mM; sodium dodecyl sulfate 75 mM; sodium phosphate 25 mM; sodium azide 0.05 % (w/v); H2O 93%; D2O 7%
sample_2: Envelope polyprotein GP160, [U-15N], 0.7 mM; sodium dodecyl sulfate 75 mM; sodium phosphate 25 mM; sodium azide 0.05 % (w/v); H2O 93%; D2O 7%
sample_3: Envelope polyprotein GP160, [U-15N], 0.7 mM; sodium dodecyl sulfate 75 mM; sodium phosphate 25 mM; sodium azide 0.05 % (w/v); H2O 93%; D2O 7%; polyacryalmide gel
sample_cond_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D HNCO | not available | not available | not available |
3D HNCA | not available | not available | not available |
3D HN(CO)CA | not available | not available | not available |
3D HN(CA)CB | not available | not available | not available |
2D HNCG | not available | not available | not available |
3D 15N-separated TOCSY | not available | not available | not available |
3D 15N-separated NOESY | not available | not available | not available |
3D HNHA | not available | not available | not available |
Software:
xwinnmr v2.6 & 3.5, Bruker - collection
NMRPipe v2.3, Delaglio - data analysis, processing
SPARKY v3.11, Goddard - data analysis
X-PLOR NIH v2.9.4, Schwieters - refinement
NMR spectrometers:
- Bruker DRX 800 MHz
Related Database Links:
PDB | |
DBJ | BAD66665 BAE96228 BAE96229 BAE96230 BAE96231 |
EMBL | CAA25903 CAA43618 CAA43620 CAA77628 |
GB | AAA44205 AAA44661 AAA44679 AAA44850 AAA44992 |
PRF | 1102247A 1103299B 1923361A 2207165U 2207165V |
REF | NP_057856 NP_579895 |
SP | P03375 P04578 P04582 P04624 P19551 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
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