BMRB Entry 6915
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6915
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Title: 1H and 15N Chemical Shift Assignments of Recombinant Dendroaspin
Deposition date: 2005-12-05 Original release date: 2009-05-27
Authors: Chuang, Woei-Jer; Cheng, Chun-Ho; Chen, Yi-Chun
Citation: Chuang, Woei-Jer; Cheng, Chun-Ho; Chen, Yi-Chun. "Expression in Pichia pastoris and backbone dynamics of dendroaspin, a three finger toxin" Proteins ., .-..
Assembly members:
Dendroaspin - snake toxin, polymer, 67 residues, Formula weight is not available
Natural source: Common Name: Jameson Taxonomy ID: 8619 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Viridiplantae
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Dendroaspin - snake toxin: EFHHHHHHRICYNHLGTKPP
TTETCQEDSCYKNIWTFDNI
IRRGCGCFTPRGDMPGPYCC
ESDKCNL
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 59 |
| 1H chemical shifts | 385 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Dendroaspin monomer | 1 |
Entities:
Entity 1, Dendroaspin monomer 67 residues - Formula weight is not available
| 1 | GLU | PHE | HIS | HIS | HIS | HIS | HIS | HIS | ARG | ILE | ||||
| 2 | CYS | TYR | ASN | HIS | LEU | GLY | THR | LYS | PRO | PRO | ||||
| 3 | THR | THR | GLU | THR | CYS | GLN | GLU | ASP | SER | CYS | ||||
| 4 | TYR | LYS | ASN | ILE | TRP | THR | PHE | ASP | ASN | ILE | ||||
| 5 | ILE | ARG | ARG | GLY | CYS | GLY | CYS | PHE | THR | PRO | ||||
| 6 | ARG | GLY | ASP | MET | PRO | GLY | PRO | TYR | CYS | CYS | ||||
| 7 | GLU | SER | ASP | LYS | CYS | ASN | LEU |
Samples:
sample_1: Dendroaspin 3.18 ± 0.1 mM; H2O 90%; D2O 10%
sample_2: Dendroaspin 3.18 ± 0.1 mM; D2O 100%
sample_3: Dendroaspin, [U-98% 15N], 1.92 ± 0.01 mM; H2O 90%; D2O 10%
conditions_1: pH: 1.2; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | not available | not available | conditions_1 |
| 2D 1H-1H NOESY | not available | not available | conditions_1 |
| 2D 1H-15N HSQC | not available | not available | conditions_1 |
| 3D 1H-1H-15N TOCSY | not available | not available | conditions_1 |
| 3D 1H-1H-15N NOESY | not available | not available | conditions_1 |
| 3D HNHA | not available | not available | conditions_1 |
Software:
xwinnmr v2.6 -
AURELIA v3.0 -
NMR spectrometers:
- Bruker AVANCE 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts