BMRB Entry 6953
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6953
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Title: NMR solution of rabbit Prion Protein (91-228) PubMed: 17415669
Deposition date: 2006-01-27 Original release date: 2007-10-15
Authors: Li, J.; Lin, D.
Citation: Li, J.; Mei, F.; Xiao, G.; Lin, D.. "1H, 13C and 15N resonance assignments of rabbit prion protein (91-228)" J. Biomol. NMR 38, 181-181 (2007).
Assembly members:
Major prion protein, polymer, 138 residues, Formula weight is not available
Natural source: Common Name: Rabbit Taxonomy ID: 9986 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Oryctolagus cuniculus
Experimental source: Production method: recombiant technology
Entity Sequences (FASTA):
Major prion protein: QGGTHNQWGKPSKPKTSMKH
VAGAAAAGAVVGGLGGYMLG
SAMSRPLIHFGNDYEDRYYR
ENMYRYPNQVYYRPVDQYSN
QNSFVHDCVNITVKQHTVTT
TTKGENFTETDIKIMERVVE
QMCITQYQQESQAAYQRA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 532 |
| 15N chemical shifts | 156 |
| 1H chemical shifts | 927 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Major prion protein | 1 |
Entities:
Entity 1, Major prion protein 138 residues - Formula weight is not available
| 1 | GLN | GLY | GLY | THR | HIS | ASN | GLN | TRP | GLY | LYS | ||||
| 2 | PRO | SER | LYS | PRO | LYS | THR | SER | MET | LYS | HIS | ||||
| 3 | VAL | ALA | GLY | ALA | ALA | ALA | ALA | GLY | ALA | VAL | ||||
| 4 | VAL | GLY | GLY | LEU | GLY | GLY | TYR | MET | LEU | GLY | ||||
| 5 | SER | ALA | MET | SER | ARG | PRO | LEU | ILE | HIS | PHE | ||||
| 6 | GLY | ASN | ASP | TYR | GLU | ASP | ARG | TYR | TYR | ARG | ||||
| 7 | GLU | ASN | MET | TYR | ARG | TYR | PRO | ASN | GLN | VAL | ||||
| 8 | TYR | TYR | ARG | PRO | VAL | ASP | GLN | TYR | SER | ASN | ||||
| 9 | GLN | ASN | SER | PHE | VAL | HIS | ASP | CYS | VAL | ASN | ||||
| 10 | ILE | THR | VAL | LYS | GLN | HIS | THR | VAL | THR | THR | ||||
| 11 | THR | THR | LYS | GLY | GLU | ASN | PHE | THR | GLU | THR | ||||
| 12 | ASP | ILE | LYS | ILE | MET | GLU | ARG | VAL | VAL | GLU | ||||
| 13 | GLN | MET | CYS | ILE | THR | GLN | TYR | GLN | GLN | GLU | ||||
| 14 | SER | GLN | ALA | ALA | TYR | GLN | ARG | ALA |
Samples:
sample_1: Major prion protein, [U-15N; U-13C], 1 mM; acetate buffer 20 mM; H2O 90%; D2O 10%
sample_cond_1: pH: 4.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA-J | sample_1 | not available | sample_cond_1 |
| 3D 13C-separated NOESY | sample_1 | not available | sample_cond_1 |
| 3D 15N-separated NOESY | sample_1 | not available | sample_cond_1 |
| HNHA | sample_1 | not available | sample_cond_1 |
Software:
NMRPipe v2.1 - processing
ARIA v1.2 - refinement, structure solution
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| BMRB | 15394 15399 16328 16616 7142 |
| PDB | |
| EMBL | CBK44083 |
| GB | AAC48697 AAD01554 ABL75505 AEM44421 AEM44422 |
| REF | NP_001075490 XP_008254357 XP_008254358 |
| SP | Q95211 |
Download simulated HSQC data in one of the following formats:
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SPARKY: Backbone
or all simulated shifts