BMRB Entry 6957
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR6957
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Title: Solution structure of the SCAN homodimer from MZF-1/ZNF42 PubMed: 16950398
Deposition date: 2006-01-27 Original release date: 2006-03-29
Authors: Volkman, B.; Peterson, F.; Sander, T.; Waltner, J.
Citation: Peterson, F.; Hayes, P.; Waltner, J.; Heisner, A.; Jensen, D.; Sander, T.; Volkman, B.. "Structure of the SCAN domain from the tumor suppressor protein MZF1" J. Mol. Biol. 363, 137-147 (2006).
Assembly members:
SCAN, polymer, 94 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
SCAN: GSDPGPEAARLRFRCFHYEE
ATGPQEALAQLRELCRQWLR
PEVRSKEQMLELLVLEQFLG
ALPPEIQARVQGQRPGSPEE
AAALVDGLRREPGG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 398 |
| 15N chemical shifts | 93 |
| 1H chemical shifts | 653 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SCAN subunit 1 | 1 |
| 2 | SCAN subunit 2 | 1 |
Entities:
Entity 1, SCAN subunit 1 94 residues - Formula weight is not available
| 1 | GLY | SER | ASP | PRO | GLY | PRO | GLU | ALA | ALA | ARG | ||||
| 2 | LEU | ARG | PHE | ARG | CYS | PHE | HIS | TYR | GLU | GLU | ||||
| 3 | ALA | THR | GLY | PRO | GLN | GLU | ALA | LEU | ALA | GLN | ||||
| 4 | LEU | ARG | GLU | LEU | CYS | ARG | GLN | TRP | LEU | ARG | ||||
| 5 | PRO | GLU | VAL | ARG | SER | LYS | GLU | GLN | MET | LEU | ||||
| 6 | GLU | LEU | LEU | VAL | LEU | GLU | GLN | PHE | LEU | GLY | ||||
| 7 | ALA | LEU | PRO | PRO | GLU | ILE | GLN | ALA | ARG | VAL | ||||
| 8 | GLN | GLY | GLN | ARG | PRO | GLY | SER | PRO | GLU | GLU | ||||
| 9 | ALA | ALA | ALA | LEU | VAL | ASP | GLY | LEU | ARG | ARG | ||||
| 10 | GLU | PRO | GLY | GLY |
Samples:
sample_1: SCAN, [U-15N; U-13C], 2 mM; sodium phosphate 20 mM; NaCl 50 mM; H2O 90%; D2O 10%
sample_2: SCAN, [U-15N; U-13C], 1.7 mM; sodium phosphate 20 mM; NaCl 50 mM; H2O 90%; D2O 10%
sample_cond_1: ionic strength: 99 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | not available | not available | sample_cond_1 |
| 3D 13C-separated NOESY | not available | not available | sample_cond_1 |
| 3D 13C-separated NOESY (AROMATIC) | not available | not available | sample_cond_1 |
| 3D 13C-F1-filtered-13C-F3-separated NOESY | not available | not available | sample_cond_1 |
Software:
X-PLOR NIH v2.9.3. - refinement
xwinnmr v3.5 -
NMRPipe v2004 -
SPSCAN v1.1.0 -
XEASY v1.3 -
GARANT v2.1 -
CYANA v2.1 -
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAD92718 |
| GB | AAD55809 AAD55810 AAF80465 AAH07777 AAH53316 |
| REF | NP_001253962 NP_003413 NP_932172 XP_001099728 XP_001099833 |
| SP | P28698 |
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