BMRB Entry 6972
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6972
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Title: NMR assignment of the a' domain of thermophilic fungal protein disulfide isomerase PubMed: 16819589
Deposition date: 2006-02-03 Original release date: 2006-08-07
Authors: Nakano, Michiko; Murakami, Chiho; Yamaguchi, Yoshiki; Sasakawa, Hiroaki; Harada, Takushi; Kurimoto, Eiji; Asami, Osamu; Kajino, Tsutomu; Kato, Koichi
Citation: Nakano, Michiko; Murakami, Chiho; Yamaguchi, Yoshiki; Sasakawa, Hiroaki; Harada, Takushi; Kurimoto, Eiji; Asami, Osamu; Kajino, Tsutomu; Kato, Koichi. "NMR Assignments of the b' and a' Domains of Thermophilic Fungal Protein Disulfide Isomerase" J. Biomol. NMR 36, 44-44 (2006).
Assembly members:
PDI-a', polymer, 121 residues, Formula weight is not available
Natural source: Common Name: thermophilic fungus Taxonomy ID: 34413 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Humicola insolens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
PDI-a': GPLGSEGPVTVVVAKNYNEI
VLDDTKDVLIEFYAPWCGHC
KALAPKYEELGALYAKSEFK
DRVVIAKVDATANDVPDEIQ
GFPTIKLYPAGAKGQPVTYS
GSRTVEDLIKFIAENGKYKA
A
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 422 |
| 15N chemical shifts | 115 |
| 1H chemical shifts | 769 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PDI-a' | 1 |
Entities:
Entity 1, PDI-a' 121 residues - Formula weight is not available
| 1 | GLY | PRO | LEU | GLY | SER | GLU | GLY | PRO | VAL | THR | ||||
| 2 | VAL | VAL | VAL | ALA | LYS | ASN | TYR | ASN | GLU | ILE | ||||
| 3 | VAL | LEU | ASP | ASP | THR | LYS | ASP | VAL | LEU | ILE | ||||
| 4 | GLU | PHE | TYR | ALA | PRO | TRP | CYS | GLY | HIS | CYS | ||||
| 5 | LYS | ALA | LEU | ALA | PRO | LYS | TYR | GLU | GLU | LEU | ||||
| 6 | GLY | ALA | LEU | TYR | ALA | LYS | SER | GLU | PHE | LYS | ||||
| 7 | ASP | ARG | VAL | VAL | ILE | ALA | LYS | VAL | ASP | ALA | ||||
| 8 | THR | ALA | ASN | ASP | VAL | PRO | ASP | GLU | ILE | GLN | ||||
| 9 | GLY | PHE | PRO | THR | ILE | LYS | LEU | TYR | PRO | ALA | ||||
| 10 | GLY | ALA | LYS | GLY | GLN | PRO | VAL | THR | TYR | SER | ||||
| 11 | GLY | SER | ARG | THR | VAL | GLU | ASP | LEU | ILE | LYS | ||||
| 12 | PHE | ILE | ALA | GLU | ASN | GLY | LYS | TYR | LYS | ALA | ||||
| 13 | ALA |
Samples:
sample_1: PDI-a', [U-13C; U-15N], 1 mM
conditions_1: pH: 6.0; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N HSQC | sample_1 | not available | conditions_1 |
| 1H13C HSQC | sample_1 | not available | conditions_1 |
| HNCA | sample_1 | not available | conditions_1 |
| HN(CO)CA | sample_1 | not available | conditions_1 |
| HNCO | sample_1 | not available | conditions_1 |
| HN(CA)CO | sample_1 | not available | conditions_1 |
| CBCA(CO)NH | sample_1 | not available | conditions_1 |
| CBCANH | sample_1 | not available | conditions_1 |
| HBHA(CO)NH | sample_1 | not available | conditions_1 |
| 15N-edited TOCSY | sample_1 | not available | conditions_1 |
| H(CC)(CO)NH | sample_1 | not available | conditions_1 |
| C(CO)NH | sample_1 | not available | conditions_1 |
| HCCH-COSY | sample_1 | not available | conditions_1 |
| HCCH-TOCSY | sample_1 | not available | conditions_1 |
| 1H-1H TOCSY | sample_1 | not available | conditions_1 |
| 1H-1H NOESY | sample_1 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 800 MHz
- Bruker Avance 600 MHz
- Bruker DMX 500 MHz
Related Database Links:
| PDB |
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