BMRB Entry 6996
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6996
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Title: Phosphorylation of Cytoplasmic Tail of Tissue Factor and its Role in Modulating Structure and Binding Affinity. PubMed: 20076769
Deposition date: 2006-02-20 Original release date: 2007-06-20
Authors: Sen, Mehmet; Agrawal, Sameer; Craft, John; Ruf, Wolfram; Legge, Glen
Citation: Sen, Mehmet; Herzik, Mark; Craft, John; Creath, Andrea; Agrawal, Sameer; Ruf, Wolfram; Legge, Glen. "Spectroscopic Characterization of Successive Phosphorylation of the Tissue Factor Cytoplasmic Region" Open Spectrosc J. 3, 58-64 (2009).
Assembly members:
TFSP253, polymer, 19 residues, 2218.3 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
TFSP253: CRKAGVGQXWKENSPLNVS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 43 |
| 15N chemical shifts | 21 |
| 1H chemical shifts | 100 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TFSP253, trans conformer | 1 |
| 2 | TFSP253, cis conformer | 1 |
Entities:
Entity 1, TFSP253, trans conformer 19 residues - 2218.3 Da.
| 1 | CYS | ARG | LYS | ALA | GLY | VAL | GLY | GLN | SEP | TRP | ||||
| 2 | LYS | GLU | ASN | SER | PRO | LEU | ASN | VAL | SER |
Samples:
sample_1: TFSP253 6 ± 0.05 mM; D2O 10%
conditions_of_TFSP253: ionic strength: 0 mM; pH: 6.0; pressure: 1 atm; temperature: 285 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| TOCSY | sample_1 | not available | conditions_of_TFSP253 |
| IP-COSY | sample_1 | not available | conditions_of_TFSP253 |
| ROESY | sample_1 | not available | conditions_of_TFSP253 |
| 1H1H_NOESY | sample_1 | not available | conditions_of_TFSP253 |
| 1H15N_HSQC | sample_1 | not available | conditions_of_TFSP253 |
| 1H13C_HSQC | sample_1 | not available | conditions_of_TFSP253 |
Software:
NMRView - NMRView was used for Assignment
NMRPipe - process the Raw NMR data
NMR spectrometers:
- Bruker Avance 800 MHz
- Bruker Avance 600 MHz
Download simulated HSQC data in one of the following formats:
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SPARKY: Backbone
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