BMRB Entry 7067
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR7067
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Title: Human Nogo-A functional domain: nogo60 PubMed: 16877707
Deposition date: 2006-04-10 Original release date: 2006-11-17
Authors: Li, M.; Liu, J.; Song, J.
Citation: Li, M.; Liu, J.; Song, J.. "Nogo goes in the pure water: solution structure of Nogo-60 and design of the structured and buffer-soluble Nogo-54 for enhancing CNS regeneration" Protein Sci. 15, 1835-1841 (2006).
Assembly members:
Reticulon-4, polymer, 60 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Reticulon-4: RIYKGVIQAIQKSDEGHPFR
AYLESEVAISEELVQKYSNS
ALGHVNCTIKELRRLFLVDD
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 57 |
| 1H chemical shifts | 302 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Reticulon-4 | 1 |
Entities:
Entity 1, Reticulon-4 60 residues - Formula weight is not available
| 1 | ARG | ILE | TYR | LYS | GLY | VAL | ILE | GLN | ALA | ILE | |
| 2 | GLN | LYS | SER | ASP | GLU | GLY | HIS | PRO | PHE | ARG | |
| 3 | ALA | TYR | LEU | GLU | SER | GLU | VAL | ALA | ILE | SER | |
| 4 | GLU | GLU | LEU | VAL | GLN | LYS | TYR | SER | ASN | SER | |
| 5 | ALA | LEU | GLY | HIS | VAL | ASN | CYS | THR | ILE | LYS | |
| 6 | GLU | LEU | ARG | ARG | LEU | PHE | LEU | VAL | ASP | ASP |
Samples:
sample_1: Reticulon-4; H2O 90%; D2O 10%
sample_cond_1: pH: 4.0; pressure: 1 atm; temperature: 278 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | not available | not available | not available |
| 2D NOESY | not available | not available | not available |
| 2D TOCSY | not available | not available | not available |
Software:
DYANA vCYANA 1.0.6 - refinement
NMR spectrometers:
- Bruker INOVA 800 MHz
Related Database Links:
| BMRB | 15933 16257 |
| PDB | |
| DBJ | BAA74909 BAA83712 BAB16739 BAB18927 BAB18928 |
| EMBL | CAB71027 CAB71028 CAB71029 CAB99248 CAB99249 |
| GB | AAD27783 AAD31019 AAD31020 AAD31021 AAD31022 |
| REF | NP_001029083 NP_001068606 NP_001106692 NP_001123435 NP_001126875 |
| SP | Q99P72 Q9JK11 Q9NQC3 |
| TPG | DAA01940 DAA01967 DAA01973 DAA02035 DAA05710 |
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