BMRB Entry 7112
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7112
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Title: 1H, 13C, and 15N NMR Assignment of the Master-Rep Protein Nuclease Domain (2-95) from the Faba Bean Necrotic Yellows Virus PubMed: 17043745
Deposition date: 2006-05-17 Original release date: 2006-11-13
Authors: Vega-Rocha, Susana; Campos-Olivas, Ramon
Citation: Vega-Rocha, Susana; Gronenborn, A.; Gronenborn, B.; Campos-Olivas, R.. "1H, 13C, and 15N NMR assignment of the master Rep protein nuclease domain from the Nanovirus FBNYV" J. Biomol. NMR 38, 169-169 (2007).
Assembly members:
FBNYV_M-Rep_(2-95), polymer, 94 residues, 10825.3 Da.
Natural source: Common Name: Faba Bean Necrotic Yellows Virus Taxonomy ID: 59817 Superkingdom: Viruses Kingdom: not available Genus/species: Nanovirus Faba Bean Necrotic Yellows Virus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
FBNYV_M-Rep_(2-95): ARQVICWCFTLNNPLSPLSL
HDSMKYLVYQTEQGEAGNIH
FQGYIEMKKRTSLAGMKKLI
PGAHFEKRRGTQGEARAYSM
KEDTRLEGPWEYGE
- assigned_chemical_shifts
- coupling_constants
- heteronucl_NOEs
| Data type | Count |
| 13C chemical shifts | 415 |
| 15N chemical shifts | 99 |
| 1H chemical shifts | 665 |
| coupling constants | 70 |
| heteronuclear NOE values | 59 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | FBNYV_M-Rep_(2-95) | 1 |
Entities:
Entity 1, FBNYV_M-Rep_(2-95) 94 residues - 10825.3 Da.
| 1 | ALA | ARG | GLN | VAL | ILE | CYS | TRP | CYS | PHE | THR | ||||
| 2 | LEU | ASN | ASN | PRO | LEU | SER | PRO | LEU | SER | LEU | ||||
| 3 | HIS | ASP | SER | MET | LYS | TYR | LEU | VAL | TYR | GLN | ||||
| 4 | THR | GLU | GLN | GLY | GLU | ALA | GLY | ASN | ILE | HIS | ||||
| 5 | PHE | GLN | GLY | TYR | ILE | GLU | MET | LYS | LYS | ARG | ||||
| 6 | THR | SER | LEU | ALA | GLY | MET | LYS | LYS | LEU | ILE | ||||
| 7 | PRO | GLY | ALA | HIS | PHE | GLU | LYS | ARG | ARG | GLY | ||||
| 8 | THR | GLN | GLY | GLU | ALA | ARG | ALA | TYR | SER | MET | ||||
| 9 | LYS | GLU | ASP | THR | ARG | LEU | GLU | GLY | PRO | TRP | ||||
| 10 | GLU | TYR | GLY | GLU |
Samples:
sample_1: (2-95) M-Rep FBNYV, [U-10% 13C; U-15N], 0.5 mM; Sodium phosphate 20 mM; NaCl 600 mM; DTT 1 mM; D2O 8%
sample_2: (2-95) M-Rep FBNYV, [U-13C; U-15N], 0.5 mM; Sodium phosphate 20 mM; NaCl 600 mM; DTT 1 mM; D2O 8%
sample_3: 2-95 Rep FBNYV, [U-13C; U-15N], 0.5 mM; Sodium phosphate 20 mM; NaCl 600 mM; DTT 1 mM; D2O 100%
conditions_1: ionic strength: 1.264 M; pH: 6.6; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H15N_HSQC | not available | not available | conditions_1 |
| 2D 1H15N_HSQC long range | not available | not available | conditions_1 |
| 2D 1H13C_HSQC/HMQC | not available | not available | conditions_1 |
| 2D HNCOCG arom | not available | not available | conditions_1 |
| 2D HNCO arom | not available | not available | conditions_1 |
| 3D HNCO | not available | not available | conditions_1 |
| 3D HNCACB | not available | not available | conditions_1 |
| 3D CBCA (CO) NH | not available | not available | conditions_1 |
| 3D HNHA | not available | not available | conditions_1 |
| 3D HNHB | not available | not available | conditions_1 |
| 3D 15N edited TOCSY | not available | not available | conditions_1 |
| 3D 15N edited NOESY | not available | not available | conditions_1 |
| 3D HCACO | not available | not available | conditions_1 |
| 3D CC (CO) NH | not available | not available | conditions_1 |
| HCCH TOCSY | not available | not available | conditions_1 |
| 4D 15N, 13C NOESY | not available | not available | conditions_1 |
| 4D 13C, 13C NOESY | not available | not available | conditions_1 |
Software:
NMRView v5.0.20 -
NMRPipe v97.027.12.56 -
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 700 MHz
Related Database Links:
| BMRB | 7218 |
| PDB | |
| EMBL | CAA72209 CAB44020 |
| GB | ACX50503 AHC72237 AHC72255 AHC72263 |
| REF | NP_619567 |
| SP | O39828 Q9WIJ5 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts