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Biological Magnetic Resonance Data Bank


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BMRB Entry 7122

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7122
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Title: Structural Studies of MJ1529, an O6 Methylguanine DNA Methyltransferase   PubMed: 16826543

Deposition date: 2006-05-18 Original release date: 2007-01-11

Authors: Roberts, A.

Citation: Roberts, A.; Pelton, J.; Wemmer, D.. "Structural Studies of MJ1529, an O6 Methylguanine DNA Methyltransferase"  Magn. Reson. Chem. 44, S71-S82 (2006).

Assembly members:
Methylated-DNA--protein-cysteine methyltransferase (E.C.2.1.1.63), polymer, 167 residues, Formula weight is not available

Natural source:   Common Name: Methanocaldococcus jannaschii   Taxonomy ID: 2190   Superkingdom: Archaea   Kingdom: not available   Genus/species: Methanococcus jannaschii

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Methylated-DNA--protein-cysteine methyltransferase (E.C.2.1.1.63): MIIQIEEYFIGMIFKGNQLV RNTIPLRREEIFNFMDGEVV SNPEDEHLKVAEIILKLYFA EIDDKKVRELISYKLEVPEF TKKVLDIVKDIEFGKTLTYG DIAKKLNTSPRAVGMALKRN PLPLIIPCHRVVAKNSLGGY SYGLDKKKFILERERLNMVS FKFNKVY

Data sets:
Data typeCount
13C chemical shifts580
15N chemical shifts147
1H chemical shifts920
coupling constants101

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Methylated-DNA--protein-cysteine methyltransferase1

Entities:

Entity 1, Methylated-DNA--protein-cysteine methyltransferase 167 residues - Formula weight is not available

1   METILEILEGLNILEGLUGLUTYRPHEILE
2   GLYMETILEPHELYSGLYASNGLNLEUVAL
3   ARGASNTHRILEPROLEUARGARGGLUGLU
4   ILEPHEASNPHEMETASPGLYGLUVALVAL
5   SERASNPROGLUASPGLUHISLEULYSVAL
6   ALAGLUILEILELEULYSLEUTYRPHEALA
7   GLUILEASPASPLYSLYSVALARGGLULEU
8   ILESERTYRLYSLEUGLUVALPROGLUPHE
9   THRLYSLYSVALLEUASPILEVALLYSASP
10   ILEGLUPHEGLYLYSTHRLEUTHRTYRGLY
11   ASPILEALALYSLYSLEUASNTHRSERPRO
12   ARGALAVALGLYMETALALEULYSARGASN
13   PROLEUPROLEUILEILEPROCYSHISARG
14   VALVALALALYSASNSERLEUGLYGLYTYR
15   SERTYRGLYLEUASPLYSLYSLYSPHEILE
16   LEUGLUARGGLUARGLEUASNMETVALSER
17   PHELYSPHEASNLYSVALTYR

Samples:

sample_1: Methylated-DNA--protein-cysteine methyltransferase (E.C.2.1.1.63), [U-15N], 0.8 mM; sodium phosphate 50 mM; NaCl 500 mM; H2O 95%; D2O 5%

sample_2: Methylated-DNA--protein-cysteine methyltransferase (E.C.2.1.1.63), [U-13C; U-15N], 0.8 mM; sodium phosphate 50 mM; NaCl 500 mM; H2O 95%; D2O 5%

sample_3: Methylated-DNA--protein-cysteine methyltransferase (E.C.2.1.1.63), [U-15N], 0.8 mM; sodium phosphate 50 mM; NaCl 500 mM; D2O 100%

sample_4: Methylated-DNA--protein-cysteine methyltransferase (E.C.2.1.1.63), [U-13C], 0.8 mM; sodium phosphate 50 mM; NaCl 500 mM; H2O 94%; D2O 5%

sample_cond_1: ionic strength: 550 mM; pH: 6.2; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablesample_cond_1
HNHAnot availablenot availablesample_cond_1
4D 13C Separated NOESYnot availablenot availablesample_cond_1
4D 13C/15N Separated NOESYnot availablenot availablesample_cond_1
D20 Exchangenot availablenot availablesample_cond_1
3d 13C Separated NOESYnot availablenot availablesample_cond_1

Software:

CNS v1.1 - refinement, structure solution

NMRPipe v1 - processing

NMRView v5.0.4 - data analysis

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

PDB
GB AAB99547
REF NP_248537 WP_010871053
SP Q58924

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