BMRB Entry 7181
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7181
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Title: Solution Structure of Hypothetical protein PA4359: Northeast Structural Genomics Target PaT89
Deposition date: 2006-06-21 Original release date: 2006-11-08
Authors: Zhang, Q.; Liu, G.; Yee, A.; Arrowsmith, C.; Szyperski, T.
Citation: Zhang, Q.; Liu, G.; Yee, A.; Arrowsmith, C.; Szyperski, T.. "Solution Structure of Hypothetical protein PA4359: Northest Structural Genomics Target PaT89" . ., .-..
Assembly members:
Hypothetical protein PA4359, polymer, 75 residues, Formula weight is not available
Natural source: Common Name: Pseudomonas aeruginosa Taxonomy ID: 287 Superkingdom: Eubacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Hypothetical protein PA4359: MSALQPSRSYRITGYSPAIS
NGYRQRLFSMGLLPGAALRV
VRIAPLGDPIQVETRQTSLA
LRRKDLALLTLVPLD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 250 |
| 15N chemical shifts | 67 |
| 1H chemical shifts | 531 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Hypothetical protein PA4359 | 1 |
Entities:
Entity 1, Hypothetical protein PA4359 75 residues - Formula weight is not available
| 1 | MET | SER | ALA | LEU | GLN | PRO | SER | ARG | SER | TYR | ||||
| 2 | ARG | ILE | THR | GLY | TYR | SER | PRO | ALA | ILE | SER | ||||
| 3 | ASN | GLY | TYR | ARG | GLN | ARG | LEU | PHE | SER | MET | ||||
| 4 | GLY | LEU | LEU | PRO | GLY | ALA | ALA | LEU | ARG | VAL | ||||
| 5 | VAL | ARG | ILE | ALA | PRO | LEU | GLY | ASP | PRO | ILE | ||||
| 6 | GLN | VAL | GLU | THR | ARG | GLN | THR | SER | LEU | ALA | ||||
| 7 | LEU | ARG | ARG | LYS | ASP | LEU | ALA | LEU | LEU | THR | ||||
| 8 | LEU | VAL | PRO | LEU | ASP |
Samples:
sample_1: Hypothetical protein PA4359, [U-13C; U-15N], 0.7 mM; tris 10 mM; NaCl 300 mM; glycerol 5%; NaN3 0.01%; benzamidiine 1 mM; H2O 90%; D2O 10%
sample_cond_1: pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| GFT (4,3)D HNNCABCA | sample_1 | not available | sample_cond_1 |
| GFT (4,3)D CABCA(CO)NHN | sample_1 | not available | sample_cond_1 |
| GFT (4,3)D HABCAB(CO)NHN | sample_1 | not available | sample_cond_1 |
| GFT (4,3)D HCCH | sample_1 | not available | sample_cond_1 |
| SIMULTANEOUS HETERONUCLEAR RESOLVED [1H,1H]-NOESY | sample_1 | not available | sample_cond_1 |
Software:
DYANA v1.5 - structure solution
NMRPipe v2.3 - processing
CYANA v2.1 - structure solution
CNS v1.1 - refinement
XEASY v1.3 - data analysis
AutoStruct v2.0.0 - data analysis
UBNMR v1.0 - data analysis
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAK88116 BAP21294 BAP52855 GAA21207 GAJ55663 |
| EMBL | CAW29491 CCQ87292 CCQ87507 CDH72943 CDH79240 |
| GB | AAG07747 AAT50626 ABJ13629 ABR85041 AEO76930 |
| REF | NP_253049 WP_003093986 WP_003103819 WP_003157276 WP_015503515 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts