BMRB Entry 7209
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7209
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Title: Assignment of 1H, 13C, and 15N resonances for the PilP pilot protein from Neisseria meningitidis. PubMed: 16933020
Deposition date: 2006-07-04 Original release date: 2006-10-02
Authors: Golovanov, Alexander; Balasingham, Seetha; Tzitzilonis, Christos; Goult, Benjamin; Lian, Lu-Yun; Homberset, Havard; Tonjum, Tone; Derrick, Jeremy
Citation: Golovanov, Alexander; Balasingham, Seetha; Tzitzilonis, Christos; Goult, Benjamin; Lian, Lu-Yun; Homberset, Havard; Tonjum, Tone; Derrick, Jeremy. "Assignment of 1H, 13C, and 15N resonances for the PilP pilot protein from Neisseria meningitidis" J. Biomol. NMR 36, 68-68 (2006).
Assembly members:
PilP, polymer, 113 residues, Formula weight is not available
Natural source: Common Name: Neisseria meningitidis Taxonomy ID: 487 Superkingdom: Eubacteria Kingdom: not available Genus/species: Neisseria meningitidis
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
PilP: ETDKKGENAPDTKRIKETLE
KFSLENMRYVGILKSGQKVS
GFIEAEGYVYTVGVGNYLGQ
NYGRIESITDDSIVLNELIE
DSTGNWVSRKAELLLNSSDK
NTEQAAAPAAEQN
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 436 |
| 15N chemical shifts | 116 |
| 1H chemical shifts | 749 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PilP | 1 |
Entities:
Entity 1, PilP 113 residues - Formula weight is not available
| 1 | GLU | THR | ASP | LYS | LYS | GLY | GLU | ASN | ALA | PRO | ||||
| 2 | ASP | THR | LYS | ARG | ILE | LYS | GLU | THR | LEU | GLU | ||||
| 3 | LYS | PHE | SER | LEU | GLU | ASN | MET | ARG | TYR | VAL | ||||
| 4 | GLY | ILE | LEU | LYS | SER | GLY | GLN | LYS | VAL | SER | ||||
| 5 | GLY | PHE | ILE | GLU | ALA | GLU | GLY | TYR | VAL | TYR | ||||
| 6 | THR | VAL | GLY | VAL | GLY | ASN | TYR | LEU | GLY | GLN | ||||
| 7 | ASN | TYR | GLY | ARG | ILE | GLU | SER | ILE | THR | ASP | ||||
| 8 | ASP | SER | ILE | VAL | LEU | ASN | GLU | LEU | ILE | GLU | ||||
| 9 | ASP | SER | THR | GLY | ASN | TRP | VAL | SER | ARG | LYS | ||||
| 10 | ALA | GLU | LEU | LEU | LEU | ASN | SER | SER | ASP | LYS | ||||
| 11 | ASN | THR | GLU | GLN | ALA | ALA | ALA | PRO | ALA | ALA | ||||
| 12 | GLU | GLN | ASN |
Samples:
sample_1: PilP, [U-13C; U-15N], 0.5 – 1.0 mM; phosphate buffer 20 mM; EDTA 1 mM; H2O 95%; D2O 5%
sample_2: PilP0.5 – 1.0 mM; phosphate buffer 20 mM; EDTA 1 mM; H2O 95%; D2O 5%
conditions_1: pH: 6.1; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCO | not available | not available | conditions_1 |
| HNCACO | not available | not available | conditions_1 |
| HNCA | not available | not available | conditions_1 |
| HNCOCA | not available | not available | conditions_1 |
| HBHACONH | not available | not available | conditions_1 |
| CBCACONH | not available | not available | conditions_1 |
| HCCH-TOCSY | not available | not available | conditions_1 |
| NOESY-HSQC | not available | not available | conditions_1 |
| TOCSY-HSQC | not available | not available | conditions_1 |
| NOESY | not available | not available | conditions_1 |
| TOCSY | not available | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Bruker AVANCE DRX 600 MHz
Related Database Links:
| PDB | |
| EMBL | CAM07914 CAM09717 CAX50761 CBA04083 CBA04812 |
| GB | AAB52532 AAF42147 AAW88856 ABX72622 ACF28836 |
| REF | NP_274808 WP_002214522 WP_002217909 WP_002219788 WP_002224058 |
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