BMRB Entry 7220
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                PDB ID: 
                
                
                Entry in NMR Restraints Grid
                Validation report in NRG-CING
            Chem Shift validation:  AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7220
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Title: ephrinB2 ectodomain PubMed: 17061024
Deposition date: 2006-07-10 Original release date: 2006-11-06
Authors: Ran, Xiaoyuan; Fan, Jingsong; Song, Jianxing
Citation: Ran, Xiaoyuan; Fan, Jing-song; Song, Jianxing. "NMR assignment of Human ephrinB2 ectodomain" J. Biomol. NMR 38, 171-171 (2007).
Assembly members:
ephrinB2 ectodomain, polymer, 151 residues,  Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo Sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
ephrinB2 ectodomain: SKSIVLEPIYWNSSNSKFLP
GQGLVLYPQIGDKLDIICPK
VDSKTVGQYEYYKVYMVDKD
QADRCTIKKENTPLLNCAKP
DQDIKFTIKFQEFSPNLWGL
EFQKNKDYYIISTSNGSLEG
LDNQEGGVCQTRAMKILMKV
GQDASSAGSTR
- assigned_chemical_shifts
 
| Data type | Count | 
| 13C chemical shifts | 561 | 
| 15N chemical shifts | 154 | 
| 1H chemical shifts | 800 | 
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID | 
|---|---|---|
| 1 | ephrinB2 ectodomain monomer | 1 | 
Entities:
Entity 1, ephrinB2 ectodomain monomer 151 residues - Formula weight is not available
| 1 | SER | LYS | SER | ILE | VAL | LEU | GLU | PRO | ILE | TYR | ||||
| 2 | TRP | ASN | SER | SER | ASN | SER | LYS | PHE | LEU | PRO | ||||
| 3 | GLY | GLN | GLY | LEU | VAL | LEU | TYR | PRO | GLN | ILE | ||||
| 4 | GLY | ASP | LYS | LEU | ASP | ILE | ILE | CYS | PRO | LYS | ||||
| 5 | VAL | ASP | SER | LYS | THR | VAL | GLY | GLN | TYR | GLU | ||||
| 6 | TYR | TYR | LYS | VAL | TYR | MET | VAL | ASP | LYS | ASP | ||||
| 7 | GLN | ALA | ASP | ARG | CYS | THR | ILE | LYS | LYS | GLU | ||||
| 8 | ASN | THR | PRO | LEU | LEU | ASN | CYS | ALA | LYS | PRO | ||||
| 9 | ASP | GLN | ASP | ILE | LYS | PHE | THR | ILE | LYS | PHE | ||||
| 10 | GLN | GLU | PHE | SER | PRO | ASN | LEU | TRP | GLY | LEU | ||||
| 11 | GLU | PHE | GLN | LYS | ASN | LYS | ASP | TYR | TYR | ILE | ||||
| 12 | ILE | SER | THR | SER | ASN | GLY | SER | LEU | GLU | GLY | ||||
| 13 | LEU | ASP | ASN | GLN | GLU | GLY | GLY | VAL | CYS | GLN | ||||
| 14 | THR | ARG | ALA | MET | LYS | ILE | LEU | MET | LYS | VAL | ||||
| 15 | GLY | GLN | ASP | ALA | SER | SER | ALA | GLY | SER | THR | ||||
| 16 | ARG | 
Samples:
sample_1: ephrinB2 ectodomain, [U-13C; U-15N], 0.5 mM; phosphate buffer 20 mM
conditions_1: pH: 6.8; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions | 
|---|---|---|---|
| 1H15N_HSQC | sample_1 | not available | conditions_1 | 
| HSQC-TOCSY | sample_1 | not available | conditions_1 | 
| HNCACB | sample_1 | not available | conditions_1 | 
| CBCA(CO)NH | sample_1 | not available | conditions_1 | 
| HNCO | sample_1 | not available | conditions_1 | 
| HCCH-TOCSY | sample_1 | not available | conditions_1 | 
| (H)CC(CO)NH | sample_1 | not available | conditions_1 | 
| H(CCO)NH | sample_1 | not available | conditions_1 | 
Software:
No software information available
NMR spectrometers:
- Bruker Avance 800 MHz
 
Related Database Links:
| PDB | |
| DBJ | BAE21211 BAE21388 BAE22016 BAE24974 BAE25008 | 
| EMBL | CAJ18588 | 
| GB | AAA82934 AAA99707 AAA99708 AAC41752 AAC42052 | 
| PRF | 2108400A 2108400B | 
| REF | NP_001100798 NP_001107758 NP_004084 NP_034241 XP_001134800 | 
| SP | P52799 P52800 | 
| TPG | DAA23728 | 
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