BMRB Entry 7229
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7229
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Title: Solution structure of Brak/CXCL14 PubMed: 16987528
Deposition date: 2006-07-14 Original release date: 2007-01-04
Authors: Volkman, B.; Peterson, F.; Schwarze, S.; Harder, A.
Citation: Peterson, F.; Thorpe, J.; Harder, A.; Volkman, B.; Schwarze, S.. "Structural Determinants Involved in the Regulation of CXCL14/BRAK Expression by the 26 S Proteasome" J. Mol. Biol. 363, 813-822 (2006).
Assembly members:
Small inducible cytokine B14, polymer, 78 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Small inducible cytokine B14: GSKCKCSRKGPKIRYSDVKK
LEMKPKYPHCEEKMVIITTK
SVSRYRGQEHCLHPKLQSTK
RFIKWYNAWNEKRRVYEE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 325 |
| 15N chemical shifts | 71 |
| 1H chemical shifts | 518 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Small inducible cytokine B14 | 1 |
Entities:
Entity 1, Small inducible cytokine B14 78 residues - Formula weight is not available
| 1 | GLY | SER | LYS | CYS | LYS | CYS | SER | ARG | LYS | GLY | ||||
| 2 | PRO | LYS | ILE | ARG | TYR | SER | ASP | VAL | LYS | LYS | ||||
| 3 | LEU | GLU | MET | LYS | PRO | LYS | TYR | PRO | HIS | CYS | ||||
| 4 | GLU | GLU | LYS | MET | VAL | ILE | ILE | THR | THR | LYS | ||||
| 5 | SER | VAL | SER | ARG | TYR | ARG | GLY | GLN | GLU | HIS | ||||
| 6 | CYS | LEU | HIS | PRO | LYS | LEU | GLN | SER | THR | LYS | ||||
| 7 | ARG | PHE | ILE | LYS | TRP | TYR | ASN | ALA | TRP | ASN | ||||
| 8 | GLU | LYS | ARG | ARG | VAL | TYR | GLU | GLU |
Samples:
sample_1: Small inducible cytokine B14, [U-15N; U-13C], 1 mM; phosphate buffer 100 mM; NaCl 150 mM; H2O 95%; D2O 5%
sample_cond_1: ionic strength: 300 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | not available | not available | not available |
| 3D 13C-separated NOESY | not available | not available | not available |
| 3D 13C-separated NOESY (AROMATIC) | not available | not available | not available |
Software:
xwinnmr v3.5 - collection
NMRPipe v2004 - processing
SPSCAN v1.1.0 - data analysis
XEASY v1.3 - data analysis
GARANT v2.1 - data analysis
CYANA v2.1 - structure solution
X-PLOR NIH v2.9.3 - refinement
NMR spectrometers:
- Bruker DRX 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts