BMRB Entry 7251
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR7251
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Title: 1H chemical shift assignments for wild type p53 tetramerization domain PubMed: 18076077
Deposition date: 2006-08-08 Original release date: 2008-02-05
Authors: Carbajo, Rodrigo; Mora, Puig; Sanchez del Pino, Manuel; Perez Paya, Enrique; Pineda-Lucena, Antonio
Citation: Mora, Puig; Carbajo, Rodrigo; Pineda-Lucena, Antonio; Sanchez del Pino, Manuel; Perez Paya, Enrique. "Solvent-exposed residues located in the beta-sheet modulate the stability of the tetramerization domain of p53-A structural and combinatorial approach" Proteins 71, 1670-1685 (2008).
Assembly members:
p53TD, polymer, 31 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
p53TD: EYFTLQIRGRERFEMFRELN
EALELKDAQAG
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 229 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | subunit 1 | 1 |
| 2 | subunit 2 | 1 |
| 3 | subunit 3 | 1 |
| 4 | subunit 4 | 1 |
Entities:
Entity 1, subunit 1 31 residues - Formula weight is not available
residues 326-356 p53 tetramerization domain
| 1 | GLU | TYR | PHE | THR | LEU | GLN | ILE | ARG | GLY | ARG | ||||
| 2 | GLU | ARG | PHE | GLU | MET | PHE | ARG | GLU | LEU | ASN | ||||
| 3 | GLU | ALA | LEU | GLU | LEU | LYS | ASP | ALA | GLN | ALA | ||||
| 4 | GLY |
Samples:
sample_1: p53TD wild type 0.5 mM; phosphate buffer 40 mM
conditions_1: pH: 7.2; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H,1H NOESY | sample_1 | not available | conditions_1 |
| 1H,1H TOCSY | sample_1 | not available | conditions_1 |
Software:
TOPSPIN v1.3, Bruker - Processing
SPARKY v3.110 - Assignment
CNS v1.1, Bruker - Structure calculations
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| BMRB | 4048 6521 6522 |
| PDB | |
| DBJ | BAC16799 BAD96746 BAG35463 BAG59884 BAG60244 |
| EMBL | CAA25652 CAA26306 CAA34420 CAA38095 CAA42626 |
| GB | AAA17994 AAA59987 AAA59988 AAA59989 AAA61211 |
| PRF | 2006287A |
| REF | NP_000537 NP_001040616 NP_001119584 NP_001119587 NP_001119590 |
| SP | P04637 P13481 P56423 P56424 P61260 |