BMRB Entry 7266
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7266
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Title: Chemical shift assignments for protein NE1680 from Nitrosomonas europaea: Northeast Structural Genomics Consortium target NeT5
Deposition date: 2006-08-15 Original release date: 2006-09-05
Authors: Cort, John; Yee, Adelinda; Arrowsmith, Cheryl; Kennedy, Michaeal
Citation: Cort, John; Ramelot, Theresa; Yee, Adelinda; Arrowsmith, Cheryl; Kennedy, Michaeal. "Chemical shift assignments for protein NE1680 from Nitrosomonas europaea: Northeast Structural Genomics Consortium target NeT5" The BMRB entry is the only known published source for the data..
Assembly members:
NE1680, polymer, 109 residues, Formula weight is not available
Natural source: Common Name: Nitrosomonas europaea Taxonomy ID: 915 Superkingdom: Eubacteria Kingdom: Not applicable Genus/species: Nitrosomonas europaea
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
NE1680: MGSSHHHHHHSSGRENLYFQ
GHMQIHVYDTYVKAKDGHVM
HFDVFTDVRDDKKAIEFAKQ
WLSSIGEEGATVTSEECRFC
HSEKAPDEVIEAIKQNGYFI
YKMEGCNGS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 362 |
| 15N chemical shifts | 87 |
| 1H chemical shifts | 569 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | NE1680 | 1 |
Entities:
Entity 1, NE1680 109 residues - Formula weight is not available
The construct has a non-native 22-residue N-term tag sequence (MGSSHHHHHHSSGRENLYFQGH), and a two-residue C-term cloning artifact (GS).
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | ARG | GLU | ASN | LEU | TYR | PHE | GLN | ||||
| 3 | GLY | HIS | MET | GLN | ILE | HIS | VAL | TYR | ASP | THR | ||||
| 4 | TYR | VAL | LYS | ALA | LYS | ASP | GLY | HIS | VAL | MET | ||||
| 5 | HIS | PHE | ASP | VAL | PHE | THR | ASP | VAL | ARG | ASP | ||||
| 6 | ASP | LYS | LYS | ALA | ILE | GLU | PHE | ALA | LYS | GLN | ||||
| 7 | TRP | LEU | SER | SER | ILE | GLY | GLU | GLU | GLY | ALA | ||||
| 8 | THR | VAL | THR | SER | GLU | GLU | CYS | ARG | PHE | CYS | ||||
| 9 | HIS | SER | GLU | LYS | ALA | PRO | ASP | GLU | VAL | ILE | ||||
| 10 | GLU | ALA | ILE | LYS | GLN | ASN | GLY | TYR | PHE | ILE | ||||
| 11 | TYR | LYS | MET | GLU | GLY | CYS | ASN | GLY | SER |
Samples:
sample_1: NE1680, [U-13C; U-15N], 1 ± 0.2 mM; NaCl 450 ± 10 mM; MOPS 10 ± 1 mM; sodium azide 0.01 ± 0.002 w/v %; H2O 90 v/v %; D2O 10 v/v %
conditions_1: pH: 6.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N HSQC | sample_1 | not available | conditions_1 |
| 1H13C HSQC | sample_1 | not available | conditions_1 |
| HNCACB | sample_1 | not available | conditions_1 |
| CBCACONH | sample_1 | not available | conditions_1 |
| HCCH-COSY | sample_1 | not available | conditions_1 |
| HCCH-TOCSY | sample_1 | not available | conditions_1 |
| CC-NH-TOCSY | sample_1 | not available | conditions_1 |
| HC-NH-NOCSY | sample_1 | not available | conditions_1 |
| HNCO | sample_1 | not available | conditions_1 |
| HNHA | sample_1 | not available | conditions_1 |
| HBCBCGCDHD | sample_1 | not available | conditions_1 |
| CBCACOCAHA | sample_1 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Varian Inova 600 MHz
- Varian Inova 750 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts