BMRB Entry 7276
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7276
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Title: Complete 1H, 13C and 15N resonance assignments of Blo t 5, a major mite allergen from Blomia tropicalis. PubMed: 17206470
Deposition date: 2006-08-27 Original release date: 2007-01-10
Authors: Naik, Mandar; Chang, Chi-Fon; Huang, Tai-Huang
Citation: Naik, Mandar; Chang, Chi-Fon; Kuo, I-Chun; Yu, Tsunai; Fang, Pei-Ju; Chua, Kaw-Yan; Huang, Tai-Huang. "Complete 1H, 13C and 15N resonance assignments of Blo t 5, a major mite allergen from Blomia tropicalis." J. Biomol. NMR 38, 189-189 (2006).
Assembly members:
Blo t 5, polymer, 119 residues, 14035.8 Da.
Natural source: Common Name: House dust mite Taxonomy ID: 40697 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: mites and ticks Blomia tropicalis
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Blo t 5: GSQEHKPKKDDFRNEFDHLL
IEQANHAIEKGEHQLLYLQH
QLDELNENKSKELQEKIIRE
LDVVCAMIEGAQGALERELK
RTDLNILERFNYEEAQTLSK
ILLKDLKETEQKVKDIQTQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 595 |
| 15N chemical shifts | 142 |
| 1H chemical shifts | 903 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Blo t 5 | 1 |
Entities:
Entity 1, Blo t 5 119 residues - 14035.8 Da.
-2 GLY, -1 SER, 1 GLN, 2 GLU, ..., 117 GLN
| 1 | GLY | SER | GLN | GLU | HIS | LYS | PRO | LYS | LYS | ASP | ||||
| 2 | ASP | PHE | ARG | ASN | GLU | PHE | ASP | HIS | LEU | LEU | ||||
| 3 | ILE | GLU | GLN | ALA | ASN | HIS | ALA | ILE | GLU | LYS | ||||
| 4 | GLY | GLU | HIS | GLN | LEU | LEU | TYR | LEU | GLN | HIS | ||||
| 5 | GLN | LEU | ASP | GLU | LEU | ASN | GLU | ASN | LYS | SER | ||||
| 6 | LYS | GLU | LEU | GLN | GLU | LYS | ILE | ILE | ARG | GLU | ||||
| 7 | LEU | ASP | VAL | VAL | CYS | ALA | MET | ILE | GLU | GLY | ||||
| 8 | ALA | GLN | GLY | ALA | LEU | GLU | ARG | GLU | LEU | LYS | ||||
| 9 | ARG | THR | ASP | LEU | ASN | ILE | LEU | GLU | ARG | PHE | ||||
| 10 | ASN | TYR | GLU | GLU | ALA | GLN | THR | LEU | SER | LYS | ||||
| 11 | ILE | LEU | LEU | LYS | ASP | LEU | LYS | GLU | THR | GLU | ||||
| 12 | GLN | LYS | VAL | LYS | ASP | ILE | GLN | THR | GLN |
Samples:
sample_1: Blo t 5, [U-13C; U-15N], 1 ± 0.05 mM; Potassium Phosphate 50 ± 0.01 mM; Sodium Chloride 100 ± 0.01 mM; EDTA 2 ± 0.01 mM; Sodium Azide 0.001 ± 0.0001 %
sample_2: Blo t 5, [U-15N], 1 ± 0.05 mM; Potassium Phosphate 50 ± 0.01 mM; Sodium Chloride 100 ± 0.01 mM; EDTA 2 ± 0.01 mM; Sodium Azide 0.001 ± 0.0001 %
sample_3: Blo t 5 1 ± 0.05 mM; Potassium Phosphate 50 ± 0.01 mM; Sodium Chloride 100 ± 0.01 mM; EDTA 2 ± 0.01 mM; Sodium Azide 0.001 ± 0.0001 %
sample_4: Blo t 5, [U-10% 13C], 0.8 ± 0.05 mM; Potassium Phosphate 50 ± 0.01 mM; Sodium Chloride 100 ± 0.01 mM; EDTA 2 ± 0.01 mM; Sodium Azide 0.001 ± 0.0001 %
sample_5: Blo t 5, [15N]-Lys,Leu, 0.8 ± 0.05 mM; Potassium Phosphate 50 ± 0.01 mM; Sodium Chloride 100 ± 0.01 mM; EDTA 2 ± 0.01 mM; Sodium Azide 0.001 ± 0.0001 %
ph7.5_295K: pH: 7.5; temperature: 295 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N HSQC | sample_2 | not available | ph7.5_295K |
| 13C HSQC | sample_1 | not available | ph7.5_295K |
| HNCa | sample_1 | not available | ph7.5_295K |
| HN(CO)Ca | sample_1 | not available | ph7.5_295K |
| HNCaCb | sample_1 | not available | ph7.5_295K |
| CbCa(CO)NH | sample_1 | not available | ph7.5_295K |
| HNCO | sample_1 | not available | ph7.5_295K |
| HN(Ca)CO | sample_1 | not available | ph7.5_295K |
| HCaCO | sample_1 | not available | ph7.5_295K |
| HbHa(CO)NH | sample_1 | not available | ph7.5_295K |
| (H)C(CO)NH | sample_1 | not available | ph7.5_295K |
| H(CCO)NH | sample_1 | not available | ph7.5_295K |
| 15N_TocsyHSQC | sample_1 | not available | ph7.5_295K |
| HCCH-Cosy | sample_1 | not available | ph7.5_295K |
| CCH-Tocsy | sample_1 | not available | ph7.5_295K |
| HCCH-Tocsy | sample_1 | not available | ph7.5_295K |
| Cosy | sample_3 | not available | ph7.5_295K |
| 15N edited NoesyHSQC | not available | not available | ph7.5_295K |
| 13C edited NoesyHSQC | not available | not available | ph7.5_295K |
| Tocsy | sample_3 | not available | ph7.5_295K |
| Noesy | sample_3 | not available | ph7.5_295K |
| (Hb)Cb(CgCd)Hd | sample_1 | not available | ph7.5_295K |
| (Hb)Cb(CgCdCe)He | sample_1 | not available | ph7.5_295K |
| 15N HMBC | sample_2 | not available | ph7.5_295K |
| 15N HMQC | sample_2 | not available | ph7.5_295K |
| HNHa | sample_1 | not available | ph7.5_295K |
| 13C CT-HSQC | sample_4 | not available | ph7.5_295K |
Software:
xwinnmr v3.5, Bruker - NMR data acquisition, processing
SPARKY v3.112, UCSF - NMR data analysis
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 500 MHz
- Bruker Avance 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts