BMRB Entry 7404

Name: Solution structure of the U64 H/ACA snoRNA 3' terminal hairpin loop
Published: 2008-06-26

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PDB ID: 2qh3
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR7404
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Solution structure of the U64 H/ACA snoRNA 3' terminal hairpin loop PubMed: 17889661

Deposition date: 2007-08-30 Original release date: 2008-06-26

Authors: Feigon, J.; Theimer, C.; Chim, N.; Breece, K.

Citation: Theimer, C.; Jady, B.; Chim, N.; Richard, P.; Breece, K.; Kiss, T.; Feigon, J.. "Structural and functional characterization of human telomerase RNA processing and cajal body localization signals" Mol. Cell 27, 869-881 (2007).

Assembly members:
Human_U64_H-ACA_snoRNA, polymer, 23 residues, Formula weight is not available

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):
Human_U64_H-ACA_snoRNA: GGAGUGCCUUACUGUGGCAC UCC

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	127
15N chemical shifts	31
1H chemical shifts	188

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Human U64 H/ACA snoRNA	1

Entities:

Entity 1, Human U64 H/ACA snoRNA 23 residues - Formula weight is not available

1

G

A

G

U

G

C

U

2

A

C

U

G

U

G

C

A

C

3

U

C

Samples:

sample_1: telomerase_RNA_CR7 1 mM; sodium phosphate 10 mM; KCl 200 mM; EDTA 50 uM; sodium azide 0.2%; H2O 95%; D2O 5%

sample_2: telomerase_RNA_CR7, [U-13C; U-15N], 1 mM; sodium phosphate 10 mM; KCl 200 mM; EDTA 50 uM; sodium azide 0.2%; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 200 mM; pH: 6.3; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY (11echo and watergate)	sample_1	isotropic	sample_conditions_1
2D NOESY	sample_1	isotropic	sample_conditions_1
2D TOCSY	sample_1	isotropic	sample_conditions_1
Natural abundance 2D 13C HSQC	sample_1	isotropic	sample_conditions_1
2D 15N-HMQC	sample_2	isotropic	sample_conditions_1
2D 15N-CPMG-NOESY	sample_2	isotropic	sample_conditions_1
2D JNN-HNN-COSY	sample_2	isotropic	sample_conditions_1
2D 13C-HSQC	sample_2	isotropic	sample_conditions_1
2D HCCH-COCSY	sample_2	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
2D 31P spin echo difference HCCH/HSQC/HMQC	sample_2	isotropic	sample_conditions_1
2D CT-CE-HSQC	sample_2	isotropic	sample_conditions_1

Software:

xwinnmr v2.6, Bruker - collection

xwinnmr v2.6, Bruker - processing

AURELIA v3.108, Brunger - data analysis

X-PLOR v3.851, NIH - structure solution

X-PLOR v3.851, NIH - refinement

NMR spectrometers:

Bruker DRX 500 MHz
Bruker DRX 600 MHz
Bruker AVANCE 800 MHz