BMRB Entry 10028
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10028
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Title: Solution structure of the second WW domain from mouse salvador homolog 1 protein (mm45). PubMed: 17239860
Deposition date: 2006-10-20 Original release date: 2007-10-19
Authors: Ohnishi, Satoshi; Kigawa, Takanori; Koshiba, Seizo; Tomizawa, Tadashi; Sato, Manami; Tochio, Naoya; Inoue, Makoto; Harada, Takushi; Watanabe, Satoru; Yokoyama, Shigeyuki
Citation: Ohnishi, S.; Guntert, P.; Koshiba, S.; Tomizawa, T.; Akasaka, R.; Tochio, N.; Sato, M.; Inoue, M.; Harada, T.; Watanabe, S.; Tanaka, A.; Shirouzu, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of an atypical WW domain in a novel beta-clam-like dimeric form" Febs Lett. 581, 462-468 (2007).
Assembly members:
WW domain, polymer, 49 residues, Formula weight is not available
Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
WW domain: GSSGSSGPLEREGLPPGWER
VESSEFGTYYVDHTNKRAQY
RHPSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 184 |
| 15N chemical shifts | 39 |
| 1H chemical shifts | 269 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | WW domain 1 | 1 |
| 2 | WW domain 2 | 1 |
Entities:
Entity 1, WW domain 1 49 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | PRO | LEU | GLU | ||||
| 2 | ARG | GLU | GLY | LEU | PRO | PRO | GLY | TRP | GLU | ARG | ||||
| 3 | VAL | GLU | SER | SER | GLU | PHE | GLY | THR | TYR | TYR | ||||
| 4 | VAL | ASP | HIS | THR | ASN | LYS | ARG | ALA | GLN | TYR | ||||
| 5 | ARG | HIS | PRO | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: WW domain, [U-13C; U-15N], 1 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; D2O 10%; H2O 90%
condition_1: ionic strength: 0.12 M; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.935, Kobayashi, N. - data analysis
CYANA v2.2, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB |
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