BMRB Entry 10033
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10033
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Title: Soluiotn structure of J-domain of mouse DnaJ like protein
Deposition date: 2006-11-01 Original release date: 2008-08-13
Authors: Kobayashi, N.; Koshiba, S.; Inoue, M.; Tochio, N.; Tomizawa, T.; Kigawa, T.; Yokoyama, S.
Citation: Kobayashi, N.; Koshiba, S.; Inoue, M.; Tochio, N.; Tomizawa, T.; Kigawa, T.; Yokoyama, S.. "Solution Structure of J-domain of mouse DnaJ like protein" . ., .-..
Assembly members:
J-domain, polymer, 94 residues, Formula weight is not available
Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
J-domain: GSSGSSGMALEQTLKKDWYS
ILGADPSANMSDLKQKYQKL
ILLYHPDKQSADVPAGTMEE
CMQKFIEIDQAWKILGNEET
KKKYDLQRSGPSSG
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 401 |
15N chemical shifts | 92 |
1H chemical shifts | 638 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | 1700030A21Rik protein | 1 |
Entities:
Entity 1, 1700030A21Rik protein 94 residues - Formula weight is not available
1 | GLY | SER | SER | GLY | SER | SER | GLY | MET | ALA | LEU | ||||
2 | GLU | GLN | THR | LEU | LYS | LYS | ASP | TRP | TYR | SER | ||||
3 | ILE | LEU | GLY | ALA | ASP | PRO | SER | ALA | ASN | MET | ||||
4 | SER | ASP | LEU | LYS | GLN | LYS | TYR | GLN | LYS | LEU | ||||
5 | ILE | LEU | LEU | TYR | HIS | PRO | ASP | LYS | GLN | SER | ||||
6 | ALA | ASP | VAL | PRO | ALA | GLY | THR | MET | GLU | GLU | ||||
7 | CYS | MET | GLN | LYS | PHE | ILE | GLU | ILE | ASP | GLN | ||||
8 | ALA | TRP | LYS | ILE | LEU | GLY | ASN | GLU | GLU | THR | ||||
9 | LYS | LYS | LYS | TYR | ASP | LEU | GLN | ARG | SER | GLY | ||||
10 | PRO | SER | SER | GLY |
Samples:
sample_1: J-domain of DnaJ like protein, [U-13C; U-15N], 1.07 mM; Phosphate 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D 13C-separated NOESY | sample_1 | not available | condition_1 |
3D 15N-separated NOESY | sample_1 | not available | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20020425, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.901, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
PDB | |
DBJ | BAB24631 |
GB | AAH30072 AAH91774 AAK21968 EDL27758 |
REF | NP_081268 |
SP | Q91ZF0 |
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