BMRB Entry 10063
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10063
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Title: Solution structure of the fourth SH3 domain of human intersectin 2 (KIAA1256)
Deposition date: 2006-12-25 Original release date: 2008-08-14
Authors: Tochio, N.; Kigawa, T.; Koshiba, S.; Kobayashi, N.; Inoue, M.; Yokoyama, S.
Citation: Tochio, N.; Kigawa, T.; Koshiba, S.; Kobayashi, N.; Inoue, M.; Yokoyama, S.. "Solution structure of the fourth SH3 domain of human intersectin 2 (KIAA1256)" . ., .-..
Assembly members:
SH3 domain, polymer, 80 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
SH3 domain: GSSGSSGEIAQVTSAYVASG
SEQLSLAPGQLILILKKNTS
GWWQGELQARGKKRQKGWFP
ASHVKLLGPSSERASGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 304 |
| 15N chemical shifts | 74 |
| 1H chemical shifts | 482 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Intersectin 2 | 1 |
Entities:
Entity 1, Intersectin 2 80 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | GLU | ILE | ALA | |
| 2 | GLN | VAL | THR | SER | ALA | TYR | VAL | ALA | SER | GLY | |
| 3 | SER | GLU | GLN | LEU | SER | LEU | ALA | PRO | GLY | GLN | |
| 4 | LEU | ILE | LEU | ILE | LEU | LYS | LYS | ASN | THR | SER | |
| 5 | GLY | TRP | TRP | GLN | GLY | GLU | LEU | GLN | ALA | ARG | |
| 6 | GLY | LYS | LYS | ARG | GLN | LYS | GLY | TRP | PHE | PRO | |
| 7 | ALA | SER | HIS | VAL | LYS | LEU | LEU | GLY | PRO | SER | |
| 8 | SER | GLU | ARG | ALA | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: SH3 domain, [U-13C; U-15N], 1.4 mM; phosphate 20 mM; NaCl 100 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | not available | condition_1 |
| 3D 15N-separated NOESY | sample_1 | not available | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20020425, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.816, Kobayashi, N. - data analysis
CYANA v1.0.7, Guntert, P. - refinement
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB |
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