BMRB Entry 10094
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10094
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Title: Solution structure of the third CUT domain of human Homeobox protein Cux-2
Deposition date: 2007-01-24 Original release date: 2008-08-14
Authors: Nameki, N.; Tochio, N.; Koshiba, S.; Kigawa, T.; Yokoyama, S.
Citation: Nameki, N.; Tochio, N.; Koshiba, S.; Kigawa, T.; Yokoyama, S.. "Solution structure of the third CUT domain of human Homeobox protein Cux-2" . ., .-..
Assembly members:
CUT domain, polymer, 111 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
CUT domain: GSSGSSGYSGSQAPGGIQEI
VAMSPELDTYSITKRVKEVL
TDNNLGQRLFGESILGLTQG
SVSDLLSRPKPWHKLSLKGR
EPFVRMQLWLNDPHNVEKLR
DMKKLSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 462 |
| 15N chemical shifts | 103 |
| 1H chemical shifts | 734 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Homeobox protein Cux-2 | 1 |
Entities:
Entity 1, Homeobox protein Cux-2 111 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | TYR | SER | GLY | ||||
| 2 | SER | GLN | ALA | PRO | GLY | GLY | ILE | GLN | GLU | ILE | ||||
| 3 | VAL | ALA | MET | SER | PRO | GLU | LEU | ASP | THR | TYR | ||||
| 4 | SER | ILE | THR | LYS | ARG | VAL | LYS | GLU | VAL | LEU | ||||
| 5 | THR | ASP | ASN | ASN | LEU | GLY | GLN | ARG | LEU | PHE | ||||
| 6 | GLY | GLU | SER | ILE | LEU | GLY | LEU | THR | GLN | GLY | ||||
| 7 | SER | VAL | SER | ASP | LEU | LEU | SER | ARG | PRO | LYS | ||||
| 8 | PRO | TRP | HIS | LYS | LEU | SER | LEU | LYS | GLY | ARG | ||||
| 9 | GLU | PRO | PHE | VAL | ARG | MET | GLN | LEU | TRP | LEU | ||||
| 10 | ASN | ASP | PRO | HIS | ASN | VAL | GLU | LYS | LEU | ARG | ||||
| 11 | ASP | MET | LYS | LYS | LEU | SER | GLY | PRO | SER | SER | ||||
| 12 | GLY |
Samples:
sample_1: CUT domain, [U-13C; U-15N], 1.1 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | not available | condition_1 |
| 3D 13C-separated NOESY | sample_1 | not available | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20020425, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.901, Kobayashi, N. - data analysis
CYANA v1.0.7, Guntert, P. - structure solution
OPALp, Billeter, M., Guntert, P., Koradi, R. - refinement
NMR spectrometers:
- Bruker AVANCE 700 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts