BMRB Entry 10112
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10112
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Title: Solution structure of the ubiquitin domain from mouse D7Wsu128e protein
Deposition date: 2007-02-13 Original release date: 2008-08-29
Authors: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.
Citation: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the ubiquitin domain from mouse D7Wsu128e protein" Not known ., .-..
Assembly members:
D7Wsu128e protein, polymer, 95 residues, Formula weight is not available
Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
D7Wsu128e protein: GSSGSSGDAGGGVGKELVDL
KIIWNKTKHDVKVPLDSTGS
ELKQKIHSITGLPPAMQKVM
YKGLVPEDKTLREIKVTSGA
KIMVVGSTISGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 408 |
| 15N chemical shifts | 93 |
| 1H chemical shifts | 657 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | DNA segment, Chr 7, Wayne State University 128, expressed | 1 |
Entities:
Entity 1, DNA segment, Chr 7, Wayne State University 128, expressed 95 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ASP | ALA | GLY | ||||
| 2 | GLY | GLY | VAL | GLY | LYS | GLU | LEU | VAL | ASP | LEU | ||||
| 3 | LYS | ILE | ILE | TRP | ASN | LYS | THR | LYS | HIS | ASP | ||||
| 4 | VAL | LYS | VAL | PRO | LEU | ASP | SER | THR | GLY | SER | ||||
| 5 | GLU | LEU | LYS | GLN | LYS | ILE | HIS | SER | ILE | THR | ||||
| 6 | GLY | LEU | PRO | PRO | ALA | MET | GLN | LYS | VAL | MET | ||||
| 7 | TYR | LYS | GLY | LEU | VAL | PRO | GLU | ASP | LYS | THR | ||||
| 8 | LEU | ARG | GLU | ILE | LYS | VAL | THR | SER | GLY | ALA | ||||
| 9 | LYS | ILE | MET | VAL | VAL | GLY | SER | THR | ILE | SER | ||||
| 10 | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: ubiquitin domain, [U-13C; U-15N], 0.39 mM; sodium phosphate 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20020425, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.863, Kobayashi, N. - data analysis
CYANA v1.0.7, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAB30974 |
| GB | AAH11313 EDL17262 |
| REF | NP_613055 XP_006507976 XP_006507977 XP_006507978 XP_012320157 |
| SP | Q78JW9 |
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