BMRB Entry 10119
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10119
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Title: DnaJ domain of human KIAA0730 protein
Deposition date: 2007-04-02 Original release date: 2008-09-02
Authors: Kobayashi, N.; Koshiba, S.; Kigawa, T.; Yokoyama, S.
Citation: Kobayashi, N.; Koshiba, S.; Kigawa, T.; Yokoyama, S.. "Solution structure of DnaJ domain of human KIAA0730 protein" Not known ., .-..
Assembly members:
DnaJ domain, polymer, 88 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
DnaJ domain: MHHHHHHLVPRGSILKEVTS
VVEQAWKLPESERKKIIRRL
YLKWHPDKNPENHDIANEVF
KHLQNEINRLEKQAFLDQNA
DRASRRTF
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 371 |
| 15N chemical shifts | 90 |
| 1H chemical shifts | 617 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | KIAA0730 protein | 1 |
Entities:
Entity 1, KIAA0730 protein 88 residues - Formula weight is not available
| 1 | MET | HIS | HIS | HIS | HIS | HIS | HIS | LEU | VAL | PRO | ||||
| 2 | ARG | GLY | SER | ILE | LEU | LYS | GLU | VAL | THR | SER | ||||
| 3 | VAL | VAL | GLU | GLN | ALA | TRP | LYS | LEU | PRO | GLU | ||||
| 4 | SER | GLU | ARG | LYS | LYS | ILE | ILE | ARG | ARG | LEU | ||||
| 5 | TYR | LEU | LYS | TRP | HIS | PRO | ASP | LYS | ASN | PRO | ||||
| 6 | GLU | ASN | HIS | ASP | ILE | ALA | ASN | GLU | VAL | PHE | ||||
| 7 | LYS | HIS | LEU | GLN | ASN | GLU | ILE | ASN | ARG | LEU | ||||
| 8 | GLU | LYS | GLN | ALA | PHE | LEU | ASP | GLN | ASN | ALA | ||||
| 9 | ASP | ARG | ALA | SER | ARG | ARG | THR | PHE |
Samples:
sample_1: DnaJ domain, [U-13C; U-15N], 1.3 mM; Sodium Phosphate 20 mM; Sodium chloride 100 mM; Sodium azide 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 100 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v1.8, Delaglio, F. - processing
NMRView v5.03, Johnson - data analysis
DYANA, Guntert - structure solution
CNS v1.1, Brunger - refinement
NMR spectrometers:
- Bruker AVANCE 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAA34450 BAB39340 BAD32289 |
| GB | AAF31262 AAF31263 EAX08322 EDL36107 EDL85271 |
| REF | NP_001264984 NP_055178 XP_001089964 XP_002824122 XP_003477292 |
| SP | Q9JLC8 Q9NZJ4 |
| TPG | DAA23892 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts