BMRB Entry 10241
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10241
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Title: Solution structures of the SH3 domain of human rho guanine exchange factor (GEF) 16
Deposition date: 2008-10-24 Original release date: 2009-11-03
Authors: Sato, M.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Sato, M.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structures of the SH3 domain of human rho guanine exchange factor (GEF) 16" . ., .-..
Assembly members:
SH3 domain, polymer, 79 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
SH3 domain: GSSGSSGWQGLSSKGDLPQV
EITKAFFAKQADEVTLQQAD
VVLVLQQEDGWLYGERLRDG
ETGWFPEDFARFISGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 319 |
| 15N chemical shifts | 77 |
| 1H chemical shifts | 498 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SH3 domain | 1 |
Entities:
Entity 1, SH3 domain 79 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | TRP | GLN | GLY | ||||
| 2 | LEU | SER | SER | LYS | GLY | ASP | LEU | PRO | GLN | VAL | ||||
| 3 | GLU | ILE | THR | LYS | ALA | PHE | PHE | ALA | LYS | GLN | ||||
| 4 | ALA | ASP | GLU | VAL | THR | LEU | GLN | GLN | ALA | ASP | ||||
| 5 | VAL | VAL | LEU | VAL | LEU | GLN | GLN | GLU | ASP | GLY | ||||
| 6 | TRP | LEU | TYR | GLY | GLU | ARG | LEU | ARG | ASP | GLY | ||||
| 7 | GLU | THR | GLY | TRP | PHE | PRO | GLU | ASP | PHE | ALA | ||||
| 8 | ARG | PHE | ILE | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: SH3 domain, [U-13C; U-15N], 1 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9295, Kobayashi, N. - data analysis
CYANA v1.0.7, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAA13745 BAG53186 BAG58889 |
| GB | AAH02681 AAH51838 AAP35934 AAP36764 AAX29017 |
| REF | NP_055263 XP_004024598 XP_008968944 XP_009234043 XP_009445318 |
| SP | Q5VV41 |
Download simulated HSQC data in one of the following formats:
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SPARKY: Backbone
or all simulated shifts