BMRB Entry 10254
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10254
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution structure of the C-terminal PH domain of FYVE, RhoGEF and PH domain containing protein 3 (FGD3) from human
Deposition date: 2008-11-20 Original release date: 2009-11-20
Authors: Li, H.; Tomizawa, T.; Tochio, N.; Muto, Y.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Li, H.; Tomizawa, T.; Tochio, N.; Muto, Y.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the C-terminal PH domain of FYVE, RhoGEF and PH domain containing protein 3 (FGD3) from human" . ., .-..
Assembly members:
PH domain, polymer, 112 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
PH domain: GSSGSSGSLLCGPLRLSESG
ETWSEVWAAIPMSDPQVLHL
QGGSQDGRLPRTIPLPSCKL
SVPDPEERLDSGHVWKLQWA
KQSWYLSASSAELQQQWLET
LSTAAHSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 454 |
| 15N chemical shifts | 108 |
| 1H chemical shifts | 711 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PH domain | 1 |
Entities:
Entity 1, PH domain 112 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | SER | LEU | LEU | ||||
| 2 | CYS | GLY | PRO | LEU | ARG | LEU | SER | GLU | SER | GLY | ||||
| 3 | GLU | THR | TRP | SER | GLU | VAL | TRP | ALA | ALA | ILE | ||||
| 4 | PRO | MET | SER | ASP | PRO | GLN | VAL | LEU | HIS | LEU | ||||
| 5 | GLN | GLY | GLY | SER | GLN | ASP | GLY | ARG | LEU | PRO | ||||
| 6 | ARG | THR | ILE | PRO | LEU | PRO | SER | CYS | LYS | LEU | ||||
| 7 | SER | VAL | PRO | ASP | PRO | GLU | GLU | ARG | LEU | ASP | ||||
| 8 | SER | GLY | HIS | VAL | TRP | LYS | LEU | GLN | TRP | ALA | ||||
| 9 | LYS | GLN | SER | TRP | TYR | LEU | SER | ALA | SER | SER | ||||
| 10 | ALA | GLU | LEU | GLN | GLN | GLN | TRP | LEU | GLU | THR | ||||
| 11 | LEU | SER | THR | ALA | ALA | HIS | SER | GLY | PRO | SER | ||||
| 12 | SER | GLY |
Samples:
sample_1: PH domain, [U-13C; U-15N], 1.20 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20031121, Delaglio, F - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9295, Kobayashi, N. - data analysis
CYANA v2.0.17, Guentert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAF85365 BAG63386 BAK63162 |
| EMBL | CAH92885 |
| GB | AAH32429 AAI11055 ACE86816 ACE87503 EAW62840 |
| REF | NP_001077005 NP_001126690 NP_001273922 NP_149077 XP_003807897 |
| SP | Q5JSP0 Q5R5T1 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts