BMRB Entry 10323
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10323
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Title: Solution structure of the 13th filamin domain from human Filamin-B
Deposition date: 2009-04-02 Original release date: 2010-04-07
Authors: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.
Citation: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the 13th filamin domain from human Filamin-B" . ., .-..
Assembly members:
Filamin domain, polymer, 108 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
Filamin domain: GSSGSSGVVDPSKVKIAGPG
LGSGVRARVLQSFTVDSSKA
GLAPLEVRVLGPRGLVEPVN
VVDNGDGTHTVTYTPSQEGP
YMVSVKYADEEIPRSPFKVK
VLPTYDAS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 446 |
| 15N chemical shifts | 96 |
| 1H chemical shifts | 699 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Filamin domain | 1 |
Entities:
Entity 1, Filamin domain 108 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | VAL | VAL | ASP | ||||
| 2 | PRO | SER | LYS | VAL | LYS | ILE | ALA | GLY | PRO | GLY | ||||
| 3 | LEU | GLY | SER | GLY | VAL | ARG | ALA | ARG | VAL | LEU | ||||
| 4 | GLN | SER | PHE | THR | VAL | ASP | SER | SER | LYS | ALA | ||||
| 5 | GLY | LEU | ALA | PRO | LEU | GLU | VAL | ARG | VAL | LEU | ||||
| 6 | GLY | PRO | ARG | GLY | LEU | VAL | GLU | PRO | VAL | ASN | ||||
| 7 | VAL | VAL | ASP | ASN | GLY | ASP | GLY | THR | HIS | THR | ||||
| 8 | VAL | THR | TYR | THR | PRO | SER | GLN | GLU | GLY | PRO | ||||
| 9 | TYR | MET | VAL | SER | VAL | LYS | TYR | ALA | ASP | GLU | ||||
| 10 | GLU | ILE | PRO | ARG | SER | PRO | PHE | LYS | VAL | LYS | ||||
| 11 | VAL | LEU | PRO | THR | TYR | ASP | ALA | SER |
Samples:
sample_1: Filamin domain, [U-13C; U-15N], 1.16 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
TOPSPIN v1.3, Bruker - collection
NMRPipe v20060324, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9742, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE II 900 MHz
Related Database Links:
| PDB |
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