BMRB Entry 11098
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11098
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Title: Solution structure of the sixth fibronectin type III domain of human Netrin receptor DCC
Deposition date: 2010-02-19 Original release date: 2011-02-17
Authors: Tochio, N.; Koshiba, S.; Tomizawa, T.; Yoneyama, M.; Kigawa, T.; Yokoyama, S.
Citation: Tochio, N.; Koshiba, S.; Tomizawa, T.; Yoneyama, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the sixth fibronectin type III domain of human Netrin receptor DCC" . ., .-..
Assembly members:
sixth fn3 domain, polymer, 114 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
sixth fn3 domain: GSSGSSGPTSAPKDLTVITR
EGKPRAVIVSWQPPLEANGK
ITAYILFYTLDKNIPIDDWI
METISGDRLTHQIMDLNLDT
MYYFRIQARNSKGVGPLSDP
ILFRTLKVSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 501 |
| 15N chemical shifts | 109 |
| 1H chemical shifts | 784 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | sixth fn3 domain | 1 |
Entities:
Entity 1, sixth fn3 domain 114 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | PRO | THR | SER | ||||
| 2 | ALA | PRO | LYS | ASP | LEU | THR | VAL | ILE | THR | ARG | ||||
| 3 | GLU | GLY | LYS | PRO | ARG | ALA | VAL | ILE | VAL | SER | ||||
| 4 | TRP | GLN | PRO | PRO | LEU | GLU | ALA | ASN | GLY | LYS | ||||
| 5 | ILE | THR | ALA | TYR | ILE | LEU | PHE | TYR | THR | LEU | ||||
| 6 | ASP | LYS | ASN | ILE | PRO | ILE | ASP | ASP | TRP | ILE | ||||
| 7 | MET | GLU | THR | ILE | SER | GLY | ASP | ARG | LEU | THR | ||||
| 8 | HIS | GLN | ILE | MET | ASP | LEU | ASN | LEU | ASP | THR | ||||
| 9 | MET | TYR | TYR | PHE | ARG | ILE | GLN | ALA | ARG | ASN | ||||
| 10 | SER | LYS | GLY | VAL | GLY | PRO | LEU | SER | ASP | PRO | ||||
| 11 | ILE | LEU | PHE | ARG | THR | LEU | LYS | VAL | SER | GLY | ||||
| 12 | PRO | SER | SER | GLY |
Samples:
sample_1: sixth fn3 domain, [U-13C; U-15N], 1.3 mM; d-Tris-HCl, [U-2H], 20 mM; sodium chloride 100 mM; d-DTT, [U-2H], 1 mM; sodium azide 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-15N NOESY | sample_1 | isotropic | condition_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20031121, Delaglio, F. - processing
NMNMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.955, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB |
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