BMRB Entry 11133
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11133
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Title: Solution structure of the fourth CH domain from human L-plastin
Deposition date: 2010-03-31 Original release date: 2011-04-01
Authors: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.
Citation: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the fourth CH domain from human L-plastin" . ., .-..
Assembly members:
CH domain, polymer, 124 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
CH domain: GSSGSSGNDDIIVNWVNETL
REAEKSSSISSFKDPKISTS
LPVLDLIDAIQPGSINYDLL
KTENLNDDEKLNNAKYAISM
ARKIGARVYALPEDLVEVNP
KMVMTVFACLMGKGMKRVSG
PSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 497 |
| 15N chemical shifts | 117 |
| 1H chemical shifts | 806 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CH domain | 1 |
Entities:
Entity 1, CH domain 124 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ASN | ASP | ASP | ||||
| 2 | ILE | ILE | VAL | ASN | TRP | VAL | ASN | GLU | THR | LEU | ||||
| 3 | ARG | GLU | ALA | GLU | LYS | SER | SER | SER | ILE | SER | ||||
| 4 | SER | PHE | LYS | ASP | PRO | LYS | ILE | SER | THR | SER | ||||
| 5 | LEU | PRO | VAL | LEU | ASP | LEU | ILE | ASP | ALA | ILE | ||||
| 6 | GLN | PRO | GLY | SER | ILE | ASN | TYR | ASP | LEU | LEU | ||||
| 7 | LYS | THR | GLU | ASN | LEU | ASN | ASP | ASP | GLU | LYS | ||||
| 8 | LEU | ASN | ASN | ALA | LYS | TYR | ALA | ILE | SER | MET | ||||
| 9 | ALA | ARG | LYS | ILE | GLY | ALA | ARG | VAL | TYR | ALA | ||||
| 10 | LEU | PRO | GLU | ASP | LEU | VAL | GLU | VAL | ASN | PRO | ||||
| 11 | LYS | MET | VAL | MET | THR | VAL | PHE | ALA | CYS | LEU | ||||
| 12 | MET | GLY | LYS | GLY | MET | LYS | ARG | VAL | SER | GLY | ||||
| 13 | PRO | SER | SER | GLY |
Samples:
sample_1: CH domain, [U-13C; U-15N], 1.14 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 220 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.932, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAD97025 BAG35310 |
| GB | AAA63236 AAB02845 AAH07673 AAH10271 ABM83861 |
| REF | XP_001157685 XP_001158161 XP_002824296 XP_002824297 XP_003270105 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
or all simulated shifts