BMRB Entry 11152
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11152
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Title: Solution structure of the fibronectin type III domain of human Integrin beta-4
Deposition date: 2010-04-15 Original release date: 2011-05-05
Authors: Abe, H.; Sasagawa, A.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Abe, H.; Sasagawa, A.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the fibronectin type III domain of human Integrin beta-4" . ., .-..
Assembly members:
fn3, polymer, 118 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
fn3: GSSGSSGSHDSRLTAGVPDT
PTRLVFSALGPTSLRVSWQE
PRCERPLQGYSVEYQLLNGG
ELHRLNIPNPAQTSVVVEDL
LPNHSYVFRVRAQSQEGWGR
EREGVITIESQVSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 471 |
| 15N chemical shifts | 113 |
| 1H chemical shifts | 740 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | fn3 | 1 |
Entities:
Entity 1, fn3 118 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | SER | HIS | ASP | ||||
| 2 | SER | ARG | LEU | THR | ALA | GLY | VAL | PRO | ASP | THR | ||||
| 3 | PRO | THR | ARG | LEU | VAL | PHE | SER | ALA | LEU | GLY | ||||
| 4 | PRO | THR | SER | LEU | ARG | VAL | SER | TRP | GLN | GLU | ||||
| 5 | PRO | ARG | CYS | GLU | ARG | PRO | LEU | GLN | GLY | TYR | ||||
| 6 | SER | VAL | GLU | TYR | GLN | LEU | LEU | ASN | GLY | GLY | ||||
| 7 | GLU | LEU | HIS | ARG | LEU | ASN | ILE | PRO | ASN | PRO | ||||
| 8 | ALA | GLN | THR | SER | VAL | VAL | VAL | GLU | ASP | LEU | ||||
| 9 | LEU | PRO | ASN | HIS | SER | TYR | VAL | PHE | ARG | VAL | ||||
| 10 | ARG | ALA | GLN | SER | GLN | GLU | GLY | TRP | GLY | ARG | ||||
| 11 | GLU | ARG | GLU | GLY | VAL | ILE | THR | ILE | GLU | SER | ||||
| 12 | GLN | VAL | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: fn3, [U-13C; U-15N], 1.21 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9820, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - structure solution
NMR spectrometers:
- Bruker AVANCE 900 MHz
Related Database Links:
| PDB |
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