BMRB Entry 11154
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11154
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Title: Solution structure of the SH3 domain of Sorbin and SH3 domain-containing protein 1
Deposition date: 2010-04-15 Original release date: 2011-05-05
Authors: Abe, H.; Miyamoto, K.; Tochio, N.; Saito, K.; Kigawa, T.; Yokoyama, S.
Citation: Abe, H.; Miyamoto, K.; Tochio, N.; Saito, K.; Kigawa, T.; Yokoyama, S.. "Solution structure of the SH3 domain of Sorbin and SH3 domain-containing protein 1" . ., .-..
Assembly members:
SH3 domain, polymer, 70 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
SH3 domain: GSSGSSGGEAIAKFNFNGDT
QVEMSFRKGERITLLRQVDE
NWYEGRIPGTSRQGIFPITY
VDVISGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 277 |
| 15N chemical shifts | 64 |
| 1H chemical shifts | 432 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SH3 domain | 1 |
Entities:
Entity 1, SH3 domain 70 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | GLY | GLU | ALA | |
| 2 | ILE | ALA | LYS | PHE | ASN | PHE | ASN | GLY | ASP | THR | |
| 3 | GLN | VAL | GLU | MET | SER | PHE | ARG | LYS | GLY | GLU | |
| 4 | ARG | ILE | THR | LEU | LEU | ARG | GLN | VAL | ASP | GLU | |
| 5 | ASN | TRP | TYR | GLU | GLY | ARG | ILE | PRO | GLY | THR | |
| 6 | SER | ARG | GLN | GLY | ILE | PHE | PRO | ILE | THR | TYR | |
| 7 | VAL | ASP | VAL | ILE | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: SH3 domain, [U-13C; U-15N], 0.75 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9747, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB |
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