BMRB Entry 11159
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11159
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Title: Solution structure of the Ring finger of human Retinoblastoma-binding protein 6
Deposition date: 2010-04-15 Original release date: 2011-05-05
Authors: Abe, H.; Miyamoto, K.; Tochio, N.; Tomizawa, T.; Koshiba, S.; Harada, T.; Watanabe, S.; Kigawa, T.; Yokoyama, S.
Citation: Abe, H.; Miyamoto, K.; Tochio, N.; Tomizawa, T.; Koshiba, S.; Harada, T.; Watanabe, S.; Kigawa, T.; Yokoyama, S.. "Solution structure of the Ring finger of human Retinoblastoma-binding protein 6" . ., .-..
Assembly members:
UNP residues 249-309, C3HC4 domain, polymer, 74 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
UNP residues 249-309, C3HC4 domain: GSSGSSGEDDPIPDELLCLI
CKDIMTDAVVIPCCGNSYCD
ECIRTALLESDEHTCPTCHQ
NDVSPDALSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 276 |
| 15N chemical shifts | 63 |
| 1H chemical shifts | 429 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | UNP residues 249-309, C3HC4 domain | 1 |
| 2 | ZINC ION no.1 | 2 |
| 3 | ZINC ION no.2 | 2 |
Entities:
Entity 1, UNP residues 249-309, C3HC4 domain 74 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | GLU | ASP | ASP | ||||
| 2 | PRO | ILE | PRO | ASP | GLU | LEU | LEU | CYS | LEU | ILE | ||||
| 3 | CYS | LYS | ASP | ILE | MET | THR | ASP | ALA | VAL | VAL | ||||
| 4 | ILE | PRO | CYS | CYS | GLY | ASN | SER | TYR | CYS | ASP | ||||
| 5 | GLU | CYS | ILE | ARG | THR | ALA | LEU | LEU | GLU | SER | ||||
| 6 | ASP | GLU | HIS | THR | CYS | PRO | THR | CYS | HIS | GLN | ||||
| 7 | ASN | ASP | VAL | SER | PRO | ASP | ALA | LEU | SER | GLY | ||||
| 8 | PRO | SER | SER | GLY |
Entity 2, ZINC ION no.1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: UNP residues 249-309, C3HC4 domain, [U-13C; U-15N], 1.18 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1 mM; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9747, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| BMRB | 17466 17772 |
| PDB | |
| DBJ | BAC77636 BAC77637 BAG64162 |
| EMBL | CAA59445 |
| GB | AAB49620 AAC72432 AAH63524 AAI72357 AAL05625 |
| REF | NP_001291483 NP_008841 NP_035377 NP_061173 XP_001076339 |
| SP | P97868 Q7Z6E9 |
| TPG | DAA15466 |
Download simulated HSQC data in one of the following formats:
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