BMRB Entry 11164
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11164
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Title: Solution structure of the Zinc finger, C3HC4 type (RING finger) domain of TNF receptor-associated factor 3
Deposition date: 2010-04-15 Original release date: 2011-05-05
Authors: Abe, H.; Miyamoto, K.; Tochio, N.; Yoneyama, M.; Kigawa, T.; Yokoyama, S.
Citation: Abe, H.; Miyamoto, K.; Tochio, N.; Yoneyama, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the Zinc finger, C3HC4 type (RING finger) domain of TNF receptor-associated factor 3" . ., .-..
Assembly members:
RING-type, residues 8-66, polymer, 66 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
RING-type, residues 8-66: GSSGSSGFVKTVEDKYKCEK
CHLVLCSPKQTECGHRFCES
CMAALLSSSSPKCTACQESI
VKDKVF
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 270 |
| 15N chemical shifts | 59 |
| 1H chemical shifts | 413 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RING-type, residues 8-66 | 1 |
| 2 | ZINC ION no.1 | 2 |
| 3 | ZINC ION no.2 | 2 |
Entities:
Entity 1, RING-type, residues 8-66 66 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | PHE | VAL | LYS | ||||
| 2 | THR | VAL | GLU | ASP | LYS | TYR | LYS | CYS | GLU | LYS | ||||
| 3 | CYS | HIS | LEU | VAL | LEU | CYS | SER | PRO | LYS | GLN | ||||
| 4 | THR | GLU | CYS | GLY | HIS | ARG | PHE | CYS | GLU | SER | ||||
| 5 | CYS | MET | ALA | ALA | LEU | LEU | SER | SER | SER | SER | ||||
| 6 | PRO | LYS | CYS | THR | ALA | CYS | GLN | GLU | SER | ILE | ||||
| 7 | VAL | LYS | ASP | LYS | VAL | PHE |
Entity 2, ZINC ION no.1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: RING-type, residues 8-66, [U-13C; U-15N], 1.10 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 50 uM; IDA 1 mM; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B. A. - data analysis
Kujira v0.9747, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAH13910 BAI45792 |
| EMBL | CAD62311 |
| GB | AAA56753 AAA65732 AAA68195 AAC50112 AAH75086 |
| REF | NP_001186356 NP_003291 NP_663777 NP_663778 XP_004055781 |
| SP | Q13114 |
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