BMRB Entry 11181
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11181
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution structure of the fourth fn3 domain of KIAA1496 protein
Deposition date: 2010-07-21 Original release date: 2011-07-20
Authors: Inoue, K.; Nagashima, T.; Tochio, N.; Kigawa, T.; Hayashi, F.; Yokoyama, S.
Citation: Inoue, K.; Nagashima, T.; Tochio, N.; Kigawa, T.; Hayashi, F.; Yokoyama, S.. "Solution structure of the fourth fn3 domain of KIAA1496 protein" . ., .-..
Assembly members:
fn3 domain, polymer, 117 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
fn3 domain: GSSGSSGTVNVTTKKTPPSQ
PPGNVVWNATDTKVLLNWEQ
VKAMENESEVTGYKVFYRTS
SQNNVQVLNTNKTSAELVLP
IKEDYIIEVKATTDGGDGTS
SEQIRIPRITSSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 473 |
| 15N chemical shifts | 112 |
| 1H chemical shifts | 767 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | fn3 domain | 1 |
Entities:
Entity 1, fn3 domain 117 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | THR | VAL | ASN | ||||
| 2 | VAL | THR | THR | LYS | LYS | THR | PRO | PRO | SER | GLN | ||||
| 3 | PRO | PRO | GLY | ASN | VAL | VAL | TRP | ASN | ALA | THR | ||||
| 4 | ASP | THR | LYS | VAL | LEU | LEU | ASN | TRP | GLU | GLN | ||||
| 5 | VAL | LYS | ALA | MET | GLU | ASN | GLU | SER | GLU | VAL | ||||
| 6 | THR | GLY | TYR | LYS | VAL | PHE | TYR | ARG | THR | SER | ||||
| 7 | SER | GLN | ASN | ASN | VAL | GLN | VAL | LEU | ASN | THR | ||||
| 8 | ASN | LYS | THR | SER | ALA | GLU | LEU | VAL | LEU | PRO | ||||
| 9 | ILE | LYS | GLU | ASP | TYR | ILE | ILE | GLU | VAL | LYS | ||||
| 10 | ALA | THR | THR | ASP | GLY | GLY | ASP | GLY | THR | SER | ||||
| 11 | SER | GLU | GLN | ILE | ARG | ILE | PRO | ARG | ILE | THR | ||||
| 12 | SER | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: fn3 domain, [U-13C; U-15N], 0.98 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
VNMR v6.1C, Varian - collection
NMRPipe v20020425, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.8992, Kobayashi, N. - data analysis
CYANA v1.0.7, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Varian INOVA 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts