BMRB Entry 11185
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11185
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Title: Solution structure of the third SH3 domain of human RIM-binding protein 2
Deposition date: 2010-07-21 Original release date: 2011-07-20
Authors: Inoue, K.; Hayashi, F.; Yokoyama, S.
Citation: Inoue, K.; Hayashi, F.; Yokoyama, S.. "Solution structure of the third SH3 domain of human RIM-binding protein 2" . ., .-..
Assembly members:
SH3 domain, polymer, 76 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
SH3 domain: GSSGSSGRRMVALYDYDPRE
SSPNVDVEAELTFCTGDIIT
VFGEIDEDGFYYGELNGQKG
LVPSNFLEEVSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 298 |
| 15N chemical shifts | 69 |
| 1H chemical shifts | 456 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SH3 domain | 1 |
Entities:
Entity 1, SH3 domain 76 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ARG | ARG | MET | ||||
| 2 | VAL | ALA | LEU | TYR | ASP | TYR | ASP | PRO | ARG | GLU | ||||
| 3 | SER | SER | PRO | ASN | VAL | ASP | VAL | GLU | ALA | GLU | ||||
| 4 | LEU | THR | PHE | CYS | THR | GLY | ASP | ILE | ILE | THR | ||||
| 5 | VAL | PHE | GLY | GLU | ILE | ASP | GLU | ASP | GLY | PHE | ||||
| 6 | TYR | TYR | GLY | GLU | LEU | ASN | GLY | GLN | LYS | GLY | ||||
| 7 | LEU | VAL | PRO | SER | ASN | PHE | LEU | GLU | GLU | VAL | ||||
| 8 | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: SH3 domain, [U-13C; U-15N], 1.08 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
VNMR v6.1C, Varian - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9296, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Varian INOVA 800 MHz
Related Database Links:
| PDB |
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