BMRB Entry 11201
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11201
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Title: Solution structure of the fourth PDZ domain of KIAA1095 protein
Deposition date: 2010-07-22 Original release date: 2011-07-21
Authors: Qin, X.; Saito, K.; Kigawa, T.; Hayashi, F.; Yokoyama, S.
Citation: Qin, X.; Saito, K.; Kigawa, T.; Hayashi, F.; Yokoyama, S.. "Solution structure of the fourth PDZ domain of KIAA1095 protein" . ., .-..
Assembly members:
PDZ domain, polymer, 124 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
PDZ domain: GSSGSSGDIHQEMDREELEL
EEVDLYRMNSQDKLGLTVCY
RTDDEDDIGIYISEIDPNSI
AAKDGRIREGDRIIQINGIE
VQNREEAVALLTSEENKNFS
LLIARPELQLDEGWMDDDSG
PSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 519 |
| 15N chemical shifts | 139 |
| 1H chemical shifts | 844 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PDZ domain of KIAA1095 protein | 1 |
Entities:
Entity 1, PDZ domain of KIAA1095 protein 124 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ASP | ILE | HIS | ||||
| 2 | GLN | GLU | MET | ASP | ARG | GLU | GLU | LEU | GLU | LEU | ||||
| 3 | GLU | GLU | VAL | ASP | LEU | TYR | ARG | MET | ASN | SER | ||||
| 4 | GLN | ASP | LYS | LEU | GLY | LEU | THR | VAL | CYS | TYR | ||||
| 5 | ARG | THR | ASP | ASP | GLU | ASP | ASP | ILE | GLY | ILE | ||||
| 6 | TYR | ILE | SER | GLU | ILE | ASP | PRO | ASN | SER | ILE | ||||
| 7 | ALA | ALA | LYS | ASP | GLY | ARG | ILE | ARG | GLU | GLY | ||||
| 8 | ASP | ARG | ILE | ILE | GLN | ILE | ASN | GLY | ILE | GLU | ||||
| 9 | VAL | GLN | ASN | ARG | GLU | GLU | ALA | VAL | ALA | LEU | ||||
| 10 | LEU | THR | SER | GLU | GLU | ASN | LYS | ASN | PHE | SER | ||||
| 11 | LEU | LEU | ILE | ALA | ARG | PRO | GLU | LEU | GLN | LEU | ||||
| 12 | ASP | GLU | GLY | TRP | MET | ASP | ASP | ASP | SER | GLY | ||||
| 13 | PRO | SER | SER | GLY |
Samples:
sample_1: PDZ domain, [U-13C; U-15N], 1.10 mM; PiNa 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20020425, Delaglio.F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.854, Kobayashi, N. - data analysis
CYANA v1.0.7, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAA83047 BAC11068 BAD32376 BAE25317 BAG09968 |
| GB | AAF22131 AAF22132 AAH10329 AAI50624 AAI52418 |
| REF | NP_001258180 NP_001290068 NP_001290069 NP_001290070 NP_001290071 |
| SP | P68907 Q69ZS0 Q9UPQ7 |
| TPG | DAA17105 DAA17106 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts