BMRB Entry 11214
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11214
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Title: Solution structure of the SH3 domain of Fyn-related kinase
Deposition date: 2010-07-22 Original release date: 2011-07-21
Authors: Qin, X.; Kurosaki, C.; Yoshida, M.; Hayashi, F.; Yokoyama, S.
Citation: Qin, X.; Kurosaki, C.; Yoshida, M.; Hayashi, F.; Yokoyama, S.. "Solution structure of the SH3 domain of Fyn-related kinase" . ., .-..
Assembly members:
SH3 domain, polymer, 77 residues, Formula weight is not available
Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
SH3 domain: GSSGSSGQYFVALFDYQART
AEDLSFRAGDKLQVLDTSHE
GWWLARHLEKKGTGLGQQLQ
GYIPSNYVAEDSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 322 |
| 15N chemical shifts | 79 |
| 1H chemical shifts | 488 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SH3 domain of Fyn-related kinase | 1 |
Entities:
Entity 1, SH3 domain of Fyn-related kinase 77 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | GLN | TYR | PHE | ||||
| 2 | VAL | ALA | LEU | PHE | ASP | TYR | GLN | ALA | ARG | THR | ||||
| 3 | ALA | GLU | ASP | LEU | SER | PHE | ARG | ALA | GLY | ASP | ||||
| 4 | LYS | LEU | GLN | VAL | LEU | ASP | THR | SER | HIS | GLU | ||||
| 5 | GLY | TRP | TRP | LEU | ALA | ARG | HIS | LEU | GLU | LYS | ||||
| 6 | LYS | GLY | THR | GLY | LEU | GLY | GLN | GLN | LEU | GLN | ||||
| 7 | GLY | TYR | ILE | PRO | SER | ASN | TYR | VAL | ALA | GLU | ||||
| 8 | ASP | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: SH3 domain, [U-13C; U-15N], 1.17 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
Delta NMR v4.3, JEOL - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9296, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- JEOL ECA 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAC26155 BAC35063 BAC36718 BAE38741 |
| EMBL | CAA88658 |
| GB | AAA65197 AAH07137 EDL04891 EDL04892 EDL04893 |
| REF | NP_001153016 NP_034367 XP_006512597 XP_006512598 XP_006512599 |
| SP | Q922K9 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
or all simulated shifts